正丁酸钠对涎腺腺样囊性癌细胞株ACC-M侵袭、迁移的影响及机制研究  

The effect and mechanism of sodium butyrate on the invasion and migration in human salivary adenoid cystic carcinoma cell line ACC-M

作　　者：刘昕[1] 吴娟娟[1,2] 李萍[1] 王越[1] 李亦婕[1] 王雨[1] 魏丹[1] 宋琦[1] 

机构地区：[1]遵义医学院口腔组织病理学教研室,贵州遵义563000 [2]贵阳市口腔医院预防科,贵州贵阳550002

出　　处：《中国癌症杂志》2015年第4期281-286,共6页China Oncology

摘　　要：背景与目的:有研究证实,正丁酸钠(sodium butyrate,Na B)对肿瘤细胞具有抑制增殖、诱导分化和促凋亡的作用,但对涎腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC)的作用机制尚不明确。该研究主要探讨不同浓度Na B对SACC细胞株ACC-M侵袭、迁移的影响及其作用机制。方法:MTT法探索Na B作用ACC-M细胞的最佳浓度并观察细胞的生长情况,Transwell小室实验检测Na B对ACC-M细胞侵袭、迁移能力的影响,实时荧光定量PCR(real-time PCR,RT-PCR)和蛋白质印迹法(Western blot)分别检测5组浓度药物作用后ACC-M细胞中高迁移率蛋白-1(high mobility group box-1,HMGB1)、toll样受体-4(toll like receptor-4,TLR4)m RNA和蛋白的表达。结果:与对照组相比,加入Na B 0.625、1.25、2.5、5及10 mmol/L的5组均能抑制ACC-M细胞增殖且呈明显浓度依赖性(P<0.05)。5组浓度Na B均可抑制ACC-M细胞体外侵袭和迁移能力(P<0.05),同时能降低ACC-M细胞HMGB1、TLR4 m RNA及蛋白的表达(P<0.05);相关性分析显示TLR4蛋白表达的降低与HMGB1的抑制呈正相关(r=0.810,P<0.05)。结论:Na B可抑制ACC-M细胞增殖,显著降低ACC-M细胞的侵袭和迁移能力,同时降低HMGB1、TLR4 m RNA和蛋白的表达,且2者表达量呈明显正相关,提示Na B可能是通过下调HMGB1、TLR4 m RNA和蛋白的表达来实现对ACC-M细胞侵袭、迁移能力的抑制。Background and purpose： Researches demonstrated that the butyric acid sodium salt （sodium butyrate, NaB） has effect on the inhibition of tumor cell proliferation, differentiation and apoptosis-promoting, while the mechanism on salivary adenoid cystic carcinoma（SACC） is still uncertain. This study mainly probed into the impact of different concentration of sodium butyrate on the migration and invasion of SACC cell line ACC-M, and its mechanism of action. Methods： MTT assay explored the optimal concentration of sodium butyrate on the cell ACC-M and the observation of cell growth. Transwell assay was used to detect the effects of sodium butyrate on the ACC-M cells on the aspact of invasion and migration ability. Fluorescence real-time quantitative PCR （RT-PCR） and Western blot were used to test respectively the expression of HMGB l, TLR4 mRNA and protein in ACC-M after functioned by 5 group drugs with different concentrations. Results： Compared with the control group, on the one hand, the concentration 0.625, 1.25, 2.5, 5 and 10 mmol/L of sodium butyrate could effectively inhibit cell proliferation and apparently showing concentra- tion-dependence （P〈0.05） ; On the other hand, 5 sets concentration of sodium butyrate could also effectively inhibit invasion and migration ability of ACC-M cells in vitro （P〈0.05）, as well as reducing the expression of HMGB 1, TLR4 mRNA and protein in ACC-M cells （P〈0.05）. Furthermore related analysis showed that the decline of TLR4 protein expression was positively correlated with inhibition of HMGB1 （r=0.810, P〈0.05）. Conclusion： Sodium butyrate has an effect on inhibiting ACC-M cell proliferation, significantly reducing ACC-M cell invasion and migration capabilities, and reducing expression of HMGB 1, TLR4 mRNA and protein, and both expression amount are positively correlated, Meanwhile the positively correlation suggests that sodium butyrate probably achieve the inhibition ability by lowering the expression of HMGB1, TLR4 mRNA and protein i

关 键 词：涎腺腺样囊性癌 正丁酸钠 高迁移率蛋白-1 TOLL样受体-4 侵袭 迁移 

分 类 号：R73-37[医药卫生—肿瘤]