化学诱导性大鼠肝癌形成过程中β-catenin表达的实验研究  

The experimental study on the expression of beta-catenin in the process of rats liver cancer formation chemically

作　　者：陈海军[1] 孙梓程[1] 刘岩[1] 由广玉[2] 胡嘉航 

机构地区：[1]牡丹江市第一人民医院普外科,黑龙江牡丹江157001 [2]牡丹江市第一人民医院病理科,黑龙江牡丹江157001 [3]牡丹江市红旗医院影像科,黑龙江牡丹江157001

出　　处：《中国癌症杂志》2015年第4期300-304,共5页China Oncology

摘　　要：背景与目的:肝细胞癌(hepatocellular carcinoma,HCC)是我国常见的消化系统恶性肿瘤,其病死率较高,对HCC发生、发展及其机制的研究及治疗途径的探索是当前HCC研究的首要问题。本研究通过检测SD大鼠化学诱导HCC过程中β-catenin的表达,旨在探讨其与HCC发生、发展的关系。方法:采用复合化学诱导法诱发48只SD雄性大鼠HCC,对照组为48只SD雄性大鼠。观察大鼠生长状态,每隔3周随机处死6只大鼠,观察肝脏变化情况,取材行HE染色观察形态学改变,并采用免疫组化和蛋白印迹(Western blot法)检测β-catenin蛋白的表达情况。结果:复合化学法诱导21周后,能成功诱发大鼠HCC,免疫组化观察自第3周起大鼠肝组织中β-catenin蛋白的表达(0.27±0.01)高于正常对照组(0.21±0.02),差异有统计学意义(P<0.05),随着化学诱导时间的增加,β-catenin蛋白的表达量呈上升趋势,在第18周(0.30±0.02)和21周(0.32±0.02)时诱癌组β-catenin的表达明显增高,与前面各时间段取材组比较,差异有统计学意义(P<0.05),Western blot法结果与免疫组化结果一致。结论:在正常肝脏组织、肝硬化、肝癌的动态病变过程中β-catenin蛋白表达均不相同,表明β-catenin与HCC的发生、发展有密切关系,其可能参与HCC基因的激活。Background and purpose： Hepatocellular carcinoma （HCC） is a common malignant tumor of the digestive system in our country, with high fatality, development of HCC and the machine system research and treatment is a primary issue in current study of HCC. To explore the expression of β-catenin at different stages in the process of hepatocellular carcinoma carcinogenisis for SD rats induced by chemicals. Methods： The experimental group included 48 male SD rats mice with primary liver cancer induced by diethylnirtosamine/carbon tetrachloride/Ethanol, while 48 normal male SD rats mice were used as the control group. The rats were killed every 3 weeks to collect the specimens and observe the pathological changes by HE staining. The changes of β-catenin protein expressions were detected by immunohistochemistry and Western blot respectively. Results： SD rats liver cancer was confirmed by HE staining after 21 weeks DEN/CC14/Ethanol induction. Immunohistochemistry showed that β-catenin expression level was obviously higher in the experimental group（0.27±0.01） than that of the control group（0.21±0.02） after 3 weeks induction（P〈0.05）. As time progresses, the expression levels of β-catenin kept on rising, and at the 18th（0.30±0.02） and 21th weeks（0.32±0.02）, it was significantly higher than that of the earlier liver tissues of the experimental group（P〈0.05）, Western blot consistent with immunohistochemical results. Conclusion： β-catenin protein expression is different in the normal liver tissue, cirrhosis, liver cancer, β-catenin and the occurrence of liver carcinoma development had close relationship. β-catenin protein in the cell with further accumulation, may active a series of target gene, leading to the formation of liver cancer..

关 键 词：肝癌细胞 肝细胞癌模型 Β-CATENIN 

分 类 号：R73-3[医药卫生—肿瘤]