小脑-下丘脑通路在卒中后抑郁发病机制中的作用  

Study of the Cerebellar Hypothalamic Pathways in the Pathogenesis of Post-stroke Depression

作　　者：康笑 隋汝波[2] 张磊[2] 马贺骥[1] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121000 [2]辽宁医学院护理学院教研室,辽宁锦州121000

出　　处：《中国医科大学学报》2015年第5期389-393,399,共6页Journal of China Medical University

基　　金：国家自然科学基金专项基金(81241050);辽宁省自然科学基金(2013022018);辽宁医学院青年基金(Y2012Z014);辽宁省科学技术计划项目(2012225019)

摘　　要：目的初步证实小脑-下丘脑通路调控炎性因子参与卒中后抑郁(PSD)的发生。方法将160只雄性(SD)大鼠随机分为假手术组、卒中组、PSD组、海人酸(KA)损毁小脑顶核组、电损毁小脑上脚交叉(x SCP)组。进行行为学观察和测试,并取海马组织采用免疫组化方法分析其IL-1β和TNF-α的表达。结果与假手术组及卒中组相比,PSD组TNF-α、IL-1β含量明显增加;而KA组与x SCP组的大鼠炎性因子表达与PSD组变化一致,3组之间无统计学差异。结论小脑顶核可能通过小脑-下丘脑通路调控炎性因子进而参与卒中后抑郁的发生。Objective To preliminarily proved that the cerebellar hypothalamic pathways regulate inflammatory cytokines involved in post-stroke depression（post-stroke depression,PSD）. Methods 160 male（Sprague-Dawley,SD）rats were randomly divided into sham operation group,the stroke group,PSD group,kainic acid（kainic acid,KA）damage of cerebellar fastigial nucleus group,electrical damaged decussation of superior cer-ebellar peduncle（superior cerebellar peduncle,xSCP）group. The behavioral observation were observed and expression of IL-1βand TNF-αin hip-pocampus by immunohistochemistry. Results Compared with the sham operation group and stroke group,PSD group,TNF-α,IL-1βcontent signif-icantly increased;and the factor of rats with KA group and xSCP group expression consistent with PSD group,there was no significant difference among the three groups. Conclusion The cerebellar fastigial nuclear may through the cerebellar hypothalamic pathways regulate inflammatory cyto-kines and participate in post stroke depression.

关 键 词：卒中后抑郁 发病机制 小脑顶核 细胞因子 下丘脑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]