5,7-二甲氧基白杨素对HepG2细胞凋亡的影响研究  

Study on the effects of 5,7-dimethoxychrysin on the Apoptosis of HepG2 Cells

作　　者：罗祎敏[1] 曹建国[2] 张凯华[3] 

机构地区：[1]南华大学医学院,421001 [2]湖南师范大学医学院 [3]南华大学附属第二医院

出　　处：《长治医学院学报》2015年第2期85-88,共4页Journal of Changzhi Medical College

基　　金：2011省教育厅(11C1092);2011衡阳市科技项目(KS10)

摘　　要：目的:研究5,7-二甲氧基白杨素(dMChR)对HepG2细胞凋亡的影响。方法:体外培养HepG2细胞,分别用1.0、4.0和16.0μM的dMChR处理48h后进行AO/EB染色,用荧光显微镜进行观察;经PI染色后用流式细胞仪检测HepG2细胞发生凋亡的情况。结果:dMChR诱导HepG2细胞凋亡具有浓度依赖性,经16.0μM的dMChR处理后HepG2细胞的凋亡率最高(31.5%),与5-FU相当,其凋亡率显著高于相同浓度的白杨素组。结论:dMChR能诱导HepG2细胞凋亡。Objective：To study the effects of 5,7-dimethoxychrysin （dMChR）on the apoptosis of HepG2 cells. Methods：HepG2 cells were cultured in vitro,and treated with 1.0,4.0 and 16.0μM dMChR for 48 h,respectively.After stained with AO/EB,HepG2 cells were observed with the fluorescence microscope.After stained with PI,the apoptosis rate of HepG2 cells was detected by flow cytometry.Results：The apoptosis of HepG2 cells induced by dMChR was in a concentration dependent manner.And the highest apoptosis rate （31.5%）was obtained when HepG2 cells were treated with 1 6.0 μM dMChR,which was equivalent to 5-FU and was significantly higher than chrysin treated with the equal concentrations.Conclusion：dMChR can induce apoptosis of HepG2 cells.

关 键 词：5 7-dimethoxychrysin HEPG2 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]