慢性肝损伤兔胆石症动物模型的构建  

Establishment of rabbit cholelithiasis models caused by chronic liver injures

作　　者：王兵[1,2] 秦军[3,4] 俞渊[5] 吕震[1,2] 李辉[1,2] 金萌[1,2] 王宇[2] 唐乾利[2] 

机构地区：[1]广西中医药大学,广西南宁530001 [2]右江民族医学院,广西百色533000 [3]右江民族医学院附属玉林医院 [4]广西玉林市红十字会医院,广西玉林537000 [5]广西中医药大学第一附属医院,广西南宁530023

出　　处：《右江民族医学院学报》2015年第2期171-174,共4页Journal of Youjiang Medical University for Nationalities

基　　金：2014年广西科学研究与技术开发计划项目(桂科能14123006-3)

摘　　要：目的构建稳定、可行、可重复的兔慢性肝损伤胆石症模型。方法将90只新西兰大白兔随机分为空白对照组、肝纤维化组、肝纤维化胆石症组、肝硬化组和肝硬化胆石症组,空白对照组10只,其余各组各20只。空白对照组正常饮食,肝纤维化组、肝纤维化胆石症组予20%的CCl4-橄榄油溶液0.2ml/kg腹腔注射,肝硬化组、肝硬化胆石症组予25%的CCl4-橄榄油溶液0.3ml/kg腹腔注射,每4d1次,连续12周,进行肝组织病理学检查及Masson染色,计算结石形成率。结果肝纤维化组、肝硬化组、肝纤维化胆石症组和肝硬化胆石症组结石形成率分别为5%、10%、70%、70%,空白对照组、肝纤维化组与肝纤维化胆石症组成石率的三组间比较差异具有统计学意义(χ2=25.476,P<0.001),两两比较结果显示,空白对照组、肝纤维化组与肝纤维化胆石症组的比较差异均具有统计学意义(P1<0.001;P2<0.001);空白对照组、肝硬化组与肝硬化胆石症组成石率的三组间比较差异具有统计学意义(χ2=22.426,P<0.001),两两比较结果显示,空白对照组、肝硬化组与肝硬化胆石症组的比较差异均具有统计学意义(P1<0.001;P2<0.001)。结石主要为胆固醇结石,HE染色光镜下可见肝小叶结构遭到破坏,肝细胞肿大;Masson染色见纤维组织增多并变为蓝色,尤其在肝小叶汇管区较为明显,可见假小叶。结论通过用CCl4和高胆固醇、高热量致石饲料,能成功构建稳定、可行的兔慢性肝损伤胆石症模型,为慢性肝损伤兔胆石症实验动物模型的构建提供一定的借鉴和指导意义。Objective To establish a stable,feasible and repetitive rabbit cholelithiasis model induced by chronic liver injury. Methods Ninety New Zealand white rabbits were randomly divided into 5 groups：a blank control group （n =10）,a liver fibrosis group（n =20）,liver fibrosis cholelithiasis group（n =20）,a liv-er cirrhosis group（n =20）and a liver cirrhosis cholelithiasis group（n =20）.Among these groups,the rabbits in the blank control group was fed with normal diet,however,the rabbits of the liver fibrosis group and the liver fibrosis cholelithiasis group received 20% CCl4 - olive oil solution by intraperitoneal injection of 0.2 ml/kg,and the rabbits of the liver cirrhosis group and the liver cirrhosis cholelithiasis group received 25% CCl4 -olive oil solution by intraperitoneal injection of 0.3 ml/kg,once every 4 days for 12 weeks consecutively;then the formation rate of stone was calculated and the liver tissue histopathology examination and Masson staining were performed. Results The stone formation rates of the liver fibrosis group,of the liver cirrhosis group, of the liver fibrosis cholelithiasis group,and the liver cirrhosis cholelithiasis group was 5%,10%,70% and 70%,respectively.Tri-group comparison of stone formation rate among the blank control group,the liver fi-brosis group and the liver fibrosis cholelithiasis group yielded statistically difference （χ2 =25.476,P 〈0.001）. Group-paired comparison of stone formation rate among the blank control group,the liver fibrosis group and the liver fibrosis cholelithiasis group yielded statistically difference （P 1 〈 0.001,P 2 〈 0.001 ）;tri - group comparison of stone formation rate among the blank control group,the liver cirrhosis group and the liver cir-rhosis cholelithiasis group yielded statistically difference （χ2 =22.426,P 〈0.001）,group-paired comparison of stone formation rate among the blank control group,the liver cirrhosis group and the liver cirrhosis choleli-thiasis group yielded statistically difference （P 1 〈0.

关 键 词：肝损伤 慢性 胆结石 模型 动物 致石饲料 

分 类 号：R-332[医药卫生]