中药材中黄曲霉毒素B_1,B_2,G_1,G_2测定方法研究  

Determination Method of Aflatoxins B_1,B_2,G_1,G_2 in Chinese Medicinal Herbs

作　　者：李浩 

机构地区：[1]广西壮族自治区食品药品检验所,广西南宁530021

出　　处：《中国药业》2015年第10期57-59,共3页China Pharmaceuticals

摘　　要：目的建立测定中药材中黄曲霉毒素B1,B2,G1,G2的高效液相色谱分离-串联三重四级杆质谱分析（HPLC-MS/MS）法。方法中药材样本经70%甲醇溶液提取,并采用免疫亲和柱净化和浓缩后,以HPLC-MS/MS对4个黄曲霉毒素的含量进行测定。结果黄曲霉毒素G2和B2进样量在0.3-30 pg、黄曲霉毒素G1和B1进样量在1-100 pg范围内与峰面积呈良好线性关系（r〉0.999 0）,加样回收率为75.33%-92.29%。结论该法速度快、操作简便、灵敏度高、重复性好,适用于中药材中霉菌毒素的检测。Objective To establish a HPLC combined with electro spray ionization triple quadrupole tandem mass spectrometry（HPLC-MS/MS）method for determining aflatoxins B1,B2,G1 and G2 in Chinese medicinal herbs. Methods After extraction by 70% methanol and purification and concentration by immunoaffinity column in the Chinese medicinal herb sample,the contents of aflatoxins B1,B2,G1 and G2 were analyzed by HPLC-MS/MS. Results The linear range was 0. 3-30 pg for aflatoxins G2 and B2,1-100 pg for aflatox-ins G1 and B1 （ r ﹥ 0. 999 0 ） . The recovery rate was 75. 33% -92. 29%. Conclusion The method is accurate,simple to operate,highly sensitive,better reproducible and suitable for the aflatoxins determination of Chinese medicinal herbs.

关 键 词：中药材 黄曲霉毒素 免疫亲和柱净化 高效液相色谱分离-串联三重四级杆质谱 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]