3种实验技术在多发性骨髓瘤检测中的应用  

Application of 3 kinds of experimental techniques in detection of multiple myeloma

作　　者：芦晗 王志银[2] 李强[2] 

机构地区：[1]新乡医学院,河南新乡453000 [2]新乡医学院第一附属医院,河南新乡453100

出　　处：《检验医学与临床》2015年第9期1278-1280,共3页Laboratory Medicine and Clinic

摘　　要：目的评估形态学、免疫组化和流式细胞检测技术在多发性骨髓瘤（MM）检测中的应用价值。方法收集新乡医学院第一附属医院2013年9月至2014年5月MM患者30例,采用免疫组化和流式细胞术分析骨髓瘤细胞免疫表型,探讨其表达率与骨髓瘤细胞形态特征及相关检测指标的关系。3种检测方法组间差异采用配对样本t检验,率的比较用χ^2检验。结果形态学检测瘤细胞比例在6.00%-95.00%,免疫组化检测瘤细胞比例在9.00%-88.00%,流式细胞检测瘤细胞比例在4.07%-87.42%。形态学与免疫组化、形态学与流式细胞之间差异有统计学意义（P〈0.05）,免疫组化与流式细胞之间差异无统计学意义（P〉0.05）。结论流式细胞术、免疫组化与形态学3种技术相结合,更有助于MM的诊断及预后判断。Objective To assess the clinical value of morphology ,immunohistochemistry and flow cytometry detection techniques in the diagnosis of multiple myeloma（MM） .Methods 30 cases of MM in the First Affiliated Hospital of Xinxiang Medical College from September 2013 to May 2014 were collected for this study .The immuno‐histochemistry and flow cytometry were adopted to analyze the immunophenotyping of MM cells for investigating the relationship between the expression rates and MMcells morphological characteristics with the related detection indi‐cators .Results In the morphology examination ,the proportion of myeloma cells was 6 .00% -95 .00% ,which by u‐sing immunohistochemistry was 9 .00% -88 .00% and which by using flow cytometry was 4 .07% -87 .42% .There were statistical differences between the morphology and immunohistochemistry and between the morphology and flow cytometry（P〈0 .05） ,but there was no statistical difference between immunohistochemistry and flow cytometry（P〉0 .05） .Conclusion The combination of flow cytometry ,immunohistochemistry and morphology even more conduces to the diagnosis and prognosis judgment of MM.

关 键 词：多发性骨髓瘤 流式细胞术 免疫组化 形态 免疫表型 

分 类 号：R573.32[医药卫生—消化系统]