Anther Culture about Hybrid F_1 by Solanum torvum SW.(♂) × Solanum melongena L.(♀)  

栽培茄和水茄杂交F_1代花药离体培养的研究（英文）

作　　者：王益奎[1,2,3] 王红[1,4] 方岩岩[4] 王艳娜[1,4] 李文嘉[1] 黎炎[1] 吴永官[1] 蒋雅琴[1] 康德贤[1] 

机构地区：[1]广西农业科学院蔬菜研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007 [3]华南农业大学园艺学院,广东广州510642 [4]广西大学农学院,广西南宁530004

出　　处：《Agricultural Science & Technology》2015年第4期645-648,711,共5页农业科学与技术（英文版）

基　　金：Supported by National Science and Technology Program for Rural Development of China during the 12thFive-Year Plan Period(2012AA100103007);National Agricultural Science and Technology Achievement Transformation Fund(2013GB2E100381);Earmarked Fund for China Agriculture Research System(nycytxgxcxtd-03-10);Science and Technology Program of Guangxi(14123006-35,14123004-3-5)~~

摘　　要：Objective] This study was conducted to investigate the effects of different hormone combinations and concentrations on cal us induction and pIant regeneration of F1 hybrid of Solanum torvum SW. ＆#215; Solanum melongena L. [Method] Media suppIemented with different combinations and concentrations of hormones were pre-pared for anther cal us induction, proIiferation, differentiation and regeneration. [Re-sult] MS＋1.0 m/L 2, 4-D＋0.5 mg/L 6-BA was the optimum medium for anther cal us induction. MS＋1.0 mg/L KT＋0.1 mg/L NAA was the optimum medium for cal us proIiferation. MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA was the optimum medium for in-duction of adventitious buds. MS＋0.3 mg/L IAA was the optimum medium for root-ing of adventitious buds. The highest rooting rate was onIy 37.5 because induction of rooting was a difficuIt work. [Conclusion] The resuIts wiI provide references for outcross fertiIity and genetic improvement of eggpIant.[目的]探讨栽培茄和水茄杂种F1不育株系花药不同处理方法对愈伤组织和植株再生诱导效果。[方法]以栽培茄和水茄杂种F1的花药为再生培养对象,配制不同激素浓度组合处理,研究对杂交F1花药愈伤组织的诱导、增殖、分化及再生的影响。[结果]诱导愈伤组织最合适的激素配比为MS+1.0 mg/L 2,4-D+0.5 mg/L 6-BA;愈伤组组织增殖最合适的激素配比为MS+1.0 mg/L KT+0.1 mg/L NAA;诱导不定芽的最佳激素配比为MS+2.0 mg/L6-BA+0.3 mg/L NAA;生根率最高的激素配比为MS+0.3 mg/L IAA,因为根的诱导相对较难,生根率仅为37.5%。[结论]该研究为提高茄子远缘杂交育性及基因遗传改良奠定基础。

关 键 词：F1 hybrid Anther cuIture HORMONE 

分 类 号：S641.1[农业科学—蔬菜学]