Biochemical Evidences for Scopoletin Inhibits Ca^(2+)-ATPase Activity in the Carmine Spider Mite,Tetranychus cinnabarinus(Boisduval)  

作　　者：Qiuli HOU Dan WANG Bingchuan ZHANG Wei DING Yongqiang ZHANG 

机构地区：[1]College of Plant Protection, Southwest University [2]Institute of Pesticide, Southwest University

出　　处：《Agricultural Science & Technology》2015年第4期826-831,共6页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31272058);Specialized Research Fund for the Doctoral Program of Higher Education(20100182120021)

摘　　要：[Objective] This study aimed to investigate the acaricidal lffect of scopo- letin, and provide the biochemical evidences of scopoletin influencus Ca2＋-ATPase activity and gene expressions in the Carmine Spider Mite, Tetranych＇us cinnabarinus. in increased protein contents; kinetic analysis showed that the catalytic capability of Ca2＋-ATPase was significantly reduced by scopoletin. [Conclusion] Scopoletin exhibits a significant inhibitory effect on Ca2＋-ATPase, and its acaricidal effect against T. cinnabarinus might be due to the direct inhibition of Ca2＋-ATPase.[Objective] This study aimed to investigate the acaricidal effect of scopoletin, and provide the biochemical evidences of scopoletin influences Ca2 +-ATPase activity and gene expressions in the Carmine Spider Mite, Tetranychus cinnabarinus.[Method] The acaricidal effects of scopoletin were investigated by slip-dip method.Exposeed to different concentrations of scopoletin(0.16-2.5 mg/ml), Ca2+-ATPase activity in vivo and protein contents were investigated. For assessing the in vitro effect, Ca2+-ATPase enzyme(200 μl) prepared from normal mites were incubated with different concentrations of scopoletin reagents. [Result] Scopoletin exhibited significant inhibitory effect on Ca2+-ATPase activity both in vivo and in vitro, and resulted in increased protein contents; kinetic analysis showed that the catalytic capability of Ca2+-ATPase was significantly reduced by scopoletin. [Conclusion] Scopoletin exhibits a significant inhibitory effect on Ca2 +- ATPase, and its acaricidal effect against T. cinnabarinus might be due to the direct inhibition of Ca2+-ATPase.

关 键 词：SCOPOLETIN Ca2＋-ATPase INHIBITION Tetranychus cinnabarinus Acaricidal 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]