A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila  

作　　者：Jiang Xu Xingjie Ren Jin Sun Xia Wang Huan-Huan Qiao Bo-Wen Xu Lu-Ping Liu Jian-Quan Ni 

机构地区：[1]Gene Regulatory Lab,School of Medicine,Tsinghua University [2]Tsinghua Fly Center,Tsinghua University [3]School of Basic Medical Sciences,Wuhan University [4]College of Bioengineering,Hubei University of Technology

出　　处：《Journal of Genetics and Genomics》2015年第4期141-149,共9页遗传学报（英文版）

基　　金：supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China(No.2015BAI09B03);the National Basic Research Program(973 Program)(No.2013CB35102);the National Natural Science Foundation of China(Nos.31371496 and31301008);the Natural Science Foundation of Hubei Province(No.2013CFB031);Wuhan Youth Chenguang Program of Science and Technology(No.2014072704011259);Tsinghua-Peking Center for Life Sciences

摘　　要：The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.

关 键 词：CRISPR Genome editing DROSOPHILA Cas9 sgRNA Off-target 

分 类 号：Q963[生物学—昆虫学]