Glutathione S-transferase(GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms  

作　　者：李斐 吴惠丰 王清 李雪花 赵建民 

机构地区：[1]Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC ),Chinese Academy of Sciences [2]Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes [3]Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC) ,Chinese Academy of Sciences [4]Key Laboratory of Industrial Ecology and Environmental Engineering (MOE) ,School of Environmental Science and Technology,Dalian University of Technology

出　　处：《Chinese Journal of Oceanology and Limnology》2015年第3期705-713,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.21107136);the International Foundation for Science(No.F/5230-1)

摘　　要：Glutathione S-transferases （GSTs） are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs （VpGSTs） and four Mytilus galloprovincialis GSTs （MgGSTs） following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes （VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3） were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.Glutathione S-transferases(GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs( Vp GSTs) and four Mytilus galloprovincialis GSTs( Mg GSTs) following exposure to BDE-47. Differential expressions of the seven V p GSTs and four Mg GSTs transcripts were observed,with differences between the hepatopancreas and gills. Among these GSTs,the sigma classes( Vp GSTS1,Vp GSTS2,Vp GSTS3,Mg GST1,and Mg GST3) were highly expressed in response to BDE-47 exposure,demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of V p GSTs and Mg GSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and π were the most often observed interactions between BDE-47 and the GSTs.

关 键 词：glutathione S-transferase （GST） Venerupis philippinarum Mytilus galloprovincialis 2 2＇ 4 4＇-tetrabromodiphenyl ether （BDE-47） molecular docking BIOMARKER 

分 类 号：S917.4[农业科学—水产科学]