长期饮用牛奶对2代繁殖雌性大鼠生殖发育的影响  

Effects of Long-term Milk-drinking on Reproductive Functioning of Two-generation Female Rats

作　　者：吕艳丽[1] 赵青 王培玉[3] 张玉梅[3] 

机构地区：[1]兵器工业北京北方医院,北京100089 [2]兵器工业机关服务中心幼儿园,北京100089 [3]北京大学公共卫生学院,北京100191

出　　处：《中国食物与营养》2015年第4期75-78,共4页Food and Nutrition in China

摘　　要：目的:通过2代繁殖实验观察长期不间断饮用牛奶对雌性大鼠生殖功能的影响。方法:4w龄SD(Sprague Dawley)大鼠雌雄各20只,随机分为牛奶组及对照组(饲料能量、蛋白质含量大致相同),每组雌雄各半,10w后合笼,产下F1代后处死雄性大鼠,分别记录两代雌性大鼠的体重、器官脏器重量、动情周期、血清激素水平、各子代活产数、阴道开口时间、雌性35d肛门生殖器间距,探讨长期饮用牛奶对雌性大鼠生殖功能的影响。结果:亲代牛奶组雌鼠血液中E2(雌二醇)显著高于对照组(P<0.05),F1代牛奶组雌鼠血液中PRL(催乳素)较对照组显著升高(P<0.05);F1代第二批子鼠F2b活产数牛奶组显著低于对照组(P<0.05);其他各项指标在两代的组间差异均无统计学意义。结论:就所观察指标而言,未发现长期饮用牛奶对雌性大鼠生长发育及生殖功能有明显影响,体内激素水平和子代活产数的作用有待进一步确认。[Objective] To investigate the effect of long-term milk-drinking on reproductive functioning of generation male rats. [Method]Totally 4w Spraque-Dawley rats （20 male and 20 female） were randomly divided into milk group and control group according to their body mass and were fed with milk and normal diet for 2 generation, respectively. Indexes including body weight, number of pups, blood hormone level, reproduction organ, anogenital distance, preputial separation （PPS） and sperm parameters were examined. [Result] The E2 of milk group in P and PRL in F1 were significantly higher than control. Besides, the number of F2b in milk group was significantly lower than con- trol. There was no significant differences on the other indexes such as weight of organs, number and quality of sperm, anogenital distance and PPS between milk group and control group. [Conclusion] Long-term milk-drinking did not show significant influence on the reproductive functioning in the two-generation reproduction study. Further study was needed to determine the effects of hormone and number of pups.

关 键 词：牛奶 2代繁殖 雌鼠 生殖功能 

分 类 号：R151[医药卫生—营养与食品卫生学]