草菇泰国1号菌株交配型A因子分子遗传学及生物信息学解析  

Molecular Genetics and Bioinformatic Analysis of Mating Type A Factor of Volvariella volvace a Strain No.1 Thailand

作　　者：汪虹[1] 陈明杰[1] 冯爱萍[1] 谭琦[1] 鲍大鹏[1] 

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《食用菌学报》2015年第1期1-7,共7页Acta Edulis Fungi

基　　金：上海市科技兴农推广项目[沪农科推字(2014)第2-2号];国家科技支撑计划项目子课题(2013BAD16B02);上海市科技人才计划项目(13XD1424700)的部分研究内容

摘　　要：采用简并PCR引物获得mip基因保守片段后,通过长片段PCR技术从草菇(Volvariella volvacea)菌株泰国1号孢子单核体中扩增获得了A1、A2交配型位点的DNA序列,结果表明:每个交配型位点均含2个交配型基因(HD1和HD2),其中A1位点的交配型基因为HD1-A1-TG和HD2-A1-TG,长度分别为1341bp和1450bp,各编码446和429个氨基酸残基;A2位点的交配型基因为HD1-A2-TG和HD2-A2-TG,长度分别为1442bp和1501bp,各编码463和443个氨基酸残基。利用生物信息学方法分析了泰国1号及另外两株草菇代表性菌株V23和屏优的交配型基因编码的HD蛋白序列,结果表明:HD蛋白均含有同源异形结构域。根据系统进化树可以看出,泰国1号与V23、屏优菌株A位点HD1和HD2基因序列所编码的氨基酸序列之间亲缘关系复杂,可能具有不同的起源。DNA sequences at the A1 and A2 mating type loci of two homokaryons, each derived from single spore isolates of Volvariella volvacea No. 1 Thailand （TG）, were obtained by long-chain PCR technology using degenerate primers to identify conservative fragments of the mip （mitochondrial intermediate peptidase） gene. Each mating type locus contained two genes, HD1 and HD2. HD1-A1-T6 and HD2＂A^-T6, located at the A1 mating type locus of homokaryon A1, were 1341 bp and 1450 bp in length and encoded 446 and 429 amino acids, respectively, while HD1AzT~ and HD2AzT~, located at the A2 mating type locus of homokaryon A2, were 1442 bp and 1501 bp in length and encoded 463 and 443 amino acids, respectively. Bioinformatic analysis indicated that all the HD proteins of the two V. volvacea TG homokaryons and each of the corresponding homokaryons derived from single spore isolates of two other typical V. volvacea strains [V23 and Pingyou （PY）~ contained homeodomains, and most incorporated coiled-coil regions and nuclear localization signals. Phylogenetic analysis based on the amino acid sequences of the HD1 and HD2 proteins of the A loci of the three strains revealed close affiliations between homokaryons TG-A1 and V23-A2, homokaryons V23-A1 and PY-A2, and homokaryons PY-A1 and TG-A2.

关 键 词：草菇 A交配型基因 同源异形结构域蛋白 

分 类 号：S646.13[农业科学—蔬菜学]