高效液相色谱法测定乳粉中5种核苷酸的含量  

High Performance Liquid Chromatography Determination of 5 Nucleotides in Milk Powder

作　　者：赵贞 岳虹 刘春霞 李素琴 陈静 李翠枝 

机构地区：[1]内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010110

出　　处：《乳业科学与技术》2015年第3期26-29,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金："十二五"国家科技部支撑计划项目(2012BAK17B00)

摘　　要：建立乳粉中核苷酸高效液相色谱的测定方法。采用超纯水作为提取剂,0.5%的乙酸沉淀蛋白,SAX固相萃取小柱净化,C18色谱柱分离,流动相为0.1 mol/L的磷酸二氢钾溶液。每100 g乳粉中核苷酸添加量为0.3-50 mg时,回收率大于90%,定量限为0.3 mg/100 g。该方法具有结果准确、操作简便、重复性好等优点,可用于乳粉中核苷酸的检测。A method for the determination of nucleotides in milk powder by high performance liquid chromatography （HPLC） was developed. Nucleotides were extracted with water, deproteinated with 0.5% acetic acid and cleaned up by an SAX solid phase extraction （SPE） column. The analysis was performed on C18 column using 0.1 mol/L phosphate as the mobile phase. The recoveries of spiked samples at three levels ranging from 0.3 to 50 mg/100 g were higher than 90%. The limit of quantification was 0.3 mg/100 g. This method was accurate, simple, reproducible, and suitable for the analysis of nucleotides in milk powder.

关 键 词：高效液相色谱法 核苷酸 SAX固相萃取小柱 乳粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]