环氧化物酶-2与细胞核增殖指数在结肠癌中的表达及意义  

Expression and significance of COX-2,Ki-67 in colorectal carcinoma

作　　者：秦朝阳[1] 许京中 田胜花 段全红[4] 惠丰荷[1] 

机构地区：[1]日照市人民医院普外科,276826 [2]日照市巨峰医院内科,276812 [3]日照市中医院普外科,276800 [4]潍坊医学院外科教研室,261053

出　　处：《中华诊断学电子杂志》2015年第1期17-21,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：日照市应用技术研究与开发计划项目(2014SHSH002)

摘　　要：目的探讨环氧化物酶-2（COX-2）、细胞核增殖指数（Ki-67）在结肠癌组织中的表达及其与侵袭转移等生物学行为的关系。方法应用免疫组织化链霉菌抗生物素蛋白-过氧化物酶连结法，检测120例结肠癌、40例癌旁异型组织和20例正常结肠黏膜组织中COX-2、Ki．67的表达，比较COX-2、Ki-67的表达与结肠癌发生、分化、浸润和转移的关系及COX-2与Ki-67二者表达的相关性。采用SPSS17．0软件包，进行Fisher精确概率法及Spearman等级相关分析，COX-2、Ki-67在结肠癌中的表达等计数资料用χ2检验。结果COX-2和Ki-67在结肠癌组织中的表达（78．3％、73．3％）显著高于癌旁组织（25．0％、35．0％）和正常结肠黏膜组织（10．0％、20．0％）（COX-2：结肠癌组织与癌旁组织比较：χ2=18．755，P〈0．05；结肠癌组织与正常结肠黏膜组织比较：χ2=29．511，P〈0．05；Ki．67：结肠癌组织与癌旁组织比较：χ2=9．538，P〈0．05；结肠癌组织与正常结肠黏膜组织比较：χ2=24．621，P〈0．05）。COX-2、Ki-67在高级别与低级别结肠癌（COX-2：χ2=6．591，P〈0．05；Ki-67：χ2=6．160，P〈0．05）、不同分期（A＋B期与C＋D期比较：COX-2：χ2=4．925，P〈0．05；Ki-67：χ2=6．317，P〈0．05）、淋巴结有无转移（COX-2：χ2=6．104，P〈0．05；Ki-67;χ2=6．104，P〈0．05）不同组间表达差异有统计学意义；而在结肠癌不同组织学类型中的表达，差异无统计学意义（χ2=1．960，P〉0．05）。COX-2与Ki-67的表达正相关（r=0．323，P〈0．05）。结论COX-2、Ki-67异常表达与结肠癌的发生发展密切相关，在肠癌组织中COX-2表达上调参与肿瘤形成、发展、侵袭和转移，肠癌Ki-67增殖指数高者肿瘤侵袭、转移潜能强；COX-2、Ki-67可作为判断结肠癌生物学行为的临床参考指标。Objective To investigate the expression of cyclooxygenase 2 （COX-2） and transcription factor Ki-67 in colorectal carcinoma and their relationship with invasion and metastasis. Methods The expressions of COX-2 and Ki-67 were measured by immunohistochemical method staining in one hundred and twenty cases with colorectal carcinoma, forty cases with dysplasia tissue closely adjacent to carcinomas, and twenty cases with normal colorectal mucosa specimens. All the clinical and follow-up information were reviewed and evaluated. Fisher＇s exat test, spearman＇s correlation analysis, chi-square test were used to analyze the expressions of COX-2 and Ki-67. Results The expressions of COX-2 and Ki-67 in colorectal cancer tissues（78.3%,73.3%,respectively） were obviously higher than those in adjacent tissues （25.0%,35.0%,respectively） and normal mucosal tissues （10.0%,20.0%,respectively） （COX-2： χ2 = 18.755, P 〈0.05 ;χ2=29.511, P 〈O.05.Ki-67：χ2=9.538, P 〈0.05;χ2 =24.621, P 〈0.05）.The differences were statistically significant .The expressions of COX-2 and Ki-67 in the high grade carcinoma were higher than those in low grade cancer（ χ2= 6.591, P 〈0.05;χ2= 6.160, P 〈0.05） , and the expression in advanced cancer was significantly raised compared with early cancer （ χ2 = 4.925, P 〈 0.05 ; χ2 = 6.317, P 〈 0.05 ）. The expression levels of COX-2 were correlated with lymph node metastasis （χ2= 6.104, P〈0.05 ;χ2= 6.104, P 〈0.05） .No significant differences were found in the expressions of Ki-67 among different types of high grade cancer had no significant difference（ χ2= 0.958, P 〉0.05）,and there was no significant difference between colorectal dysplasia tissue and normal colorectal mucosa tissue. Conclusions The abnormal expressions of COX-2 and Ki-67 plays an important role in the genesis and development of colorectal carcinoma and may be responsible for the enhanced activity in tumor-induced neovascularization,invasion and metastasis. COX-2 and Ki-67 have pos

关 键 词：结肠肿瘤 环氧化酶2 细胞核增殖指数 预后 

分 类 号：R735.35[医药卫生—肿瘤]