基于吐温-姜黄素复合物的荧光探针检测脂肪酶活性  

Detection of Lipase Activity by Fluorescence Probe Based on Tween-Curcumin Complex

作　　者：汤燕[1] 张卫[1] 左焕桢 刘佳[1] 江凌 田丹碧[1] 

机构地区：[1]南京工业大学理学院,南京211816 [2]食品与轻工学院,南京211816

出　　处：《分析化学》2015年第5期658-662,共5页Chinese Journal of Analytical Chemistry

基　　金：江苏省自然科学基金(No.BK2012822);国家杰出青年科学基金(No.21225626)~~

摘　　要：本实验以姜黄素作为信号探针,吐温作为脂肪酶的底物和包裹姜黄素的载体,建立了一种检测脂肪酶活性的荧光法。实验发现,采用0.3 mmol/L吐温40,25μmol/L姜黄素,并且脂肪酶水解吐温40的时间为35 min时,姜黄素的荧光强度变化值与脂肪酶浓度在0.002~0.05 mg/m L和0.05~0.25 mg/m L范围内呈现线性关系,检出限为0.6 mg/L（S/N=3）。此探针在高通量检测脂肪酶活性以及与脂肪酶相关疾病检测领域中有较好的应用前景。A fluorescence method was established for the detection of lipase activity by using curcumin as signal probe, Tween as substrate of lipase and the carrier of curcumin. The change of fluorescence intensity showed good linear relationship with the concentrations of PPL from 0. 002- 0. 05 mg/mL and 0. 05- 0.25 mg/mL, respectively, with the detection limit of 0.6 mg/L （ S/N = 3 ）, when containing 0.3 mmol/L tween 40 and 25 μmol/L curcumin in the solution and the hydrolysis time of 35 min. There should be a great application of this method in the field of high-throughput detection of lipase activity and lipase related diseases.

关 键 词：姜黄素 吐温40 脂肪酶 荧光探针 

分 类 号：R284[医药卫生—中药学] O657.3[医药卫生—中医学]