rhKD/APPvar对实验性急性肝损伤小鼠肝细胞增殖能力的影响  

Effect of rh KD / APPvar on hepatocyte proliferation ability in mice with acute liver injury

作　　者：王虹蛟 孟威宏[2] 王强 王心童[3] 颜炜群[4] 任立群[4] 

机构地区：[1]长春解放军第461医院,长春130021 [2]沈阳军区总医院,沈阳110016 [3]吉林大学中日联谊医院,吉林130033 [4]吉林大学再生医学科学研究所,吉林130021

出　　处：《实用药物与临床》2015年第5期507-509,共3页Practical Pharmacy and Clinical Remedies

基　　金：国家自然基金课题(30300153)

摘　　要：目的探讨重组人淀粉样蛋白前体Kunitz型蛋白酶抑制剂结构域变异体(Reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant,rh KD/APPvar)对实验性急性肝损伤小鼠肝细胞增殖能力的影响。方法利用实验鼠的四氯化碳(CCl4)慢性肝损伤模型,采用免疫组化方法,检测各组增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)阳性细胞数。结果正常对照组的PCNA阳性细胞数为(2±2)个/视野,模型组的PCNA阳性细胞数为(6±3)个/视野,rh KD/APPvar小、中、大剂量组的PCNA阳性细胞个数分别为(29±110)个/视野、(38±10)个/视野、(55±12)个/视野,抑肽酶组的PCNA阳性细胞个数为(24±9)个/视野,rh KD/APP组的PCNA阳性细胞个数为(19±5)个/视野。与模型组相比,rh KD/APPvar各剂量组PCNA阳性细胞数目均显著增加(P<0.05),并且存在剂量依赖关系。rh KD/APPvar各剂量组阳性细胞数多于抑肽酶组。结论 rh KD/APPvar能够促进急性肝损伤小鼠肝细胞增殖,其作用随着剂量的增加而增强。Objective To study the effect of reorganization human Kunitz protease inhibitor domain of amy-loid protein precursor variant （ rhKD/APPvar） on hepatocyte proliferation ability in mice with experimental acute liver injury and provide theoretical basis for study on protective effect of rhKD/APPvar on acute liver injury. Methods The acute hepatic injury model of rats were induced by carbon tetrachloride （CCl4）. Immunohistochemical method was used to observe the proliferating cell nuclear antigen （ PCNA）-positive cell number in various groups. Results The positive cell number in control group was （2 ± 2）/vision;（6 ± 3）/vision in model group;（29 ± 11）/vision,（38 ± 10）/vision and （55 ± 12）/vision in low,middle,and high dose rhKD/APPvar groups; （24 ± 9）/vision in aprotinin group,（19 ± 5） /vision in rhKD/APP group. The positive cell numbers in rhKD/APPvar group markely increased compared with model group（P＆lt;0. 05）,the positive cell number increased with the increase of rhKD/APPvar. The positive cell num-bers in rhKD/APPvar groups were more than those in aprotinin group. Conclusion The rhKD/APPvar could promote the proliferation of acute injured liver cells and the effect could be enhanced by titrating of rhKD/APPvar.

关 键 词：四氯化碳 急性肝损伤 小鼠 免疫组织化学 

分 类 号：R575[医药卫生—消化系统]