膀胱癌中YAP基因的表达以及靶向干扰YAP对膀胱癌细胞株的调节作用  

Expression of YAP gene in bladder cancer and regulatory role of YAP targeted interference on bladder cancer cell

作　　者：张宁南[1] 余闫宏[1] 肖民辉[1] 章卓睿[1] 杨峻峰[1] 

机构地区：[1]云南省第一人民医院泌尿外科,云南昆明650032

出　　处：《中国现代医学杂志》2015年第13期40-42,共3页China Journal of Modern Medicine

摘　　要：目的研究膀胱癌中YAP基因的表达以及靶向干扰YAP对膀胱癌细胞株的调节作用。方法收集膀胱癌患者的膀胱癌组织和癌旁正常组织,检测YAP的表达量。培养膀胱癌T40细胞株,采用转染si RNA片段的方式靶向干扰YAP,而后检测细胞活力、细胞周期、迁移能力以及相关基因的m RNA含量。结果膀胱癌组织中YAP的m RNA和蛋白含量均高于癌旁正常组织(P<0.05)。干扰YAP组细胞的MTT值以及G0/G1期比例、S期比例低于阴性对照组,G2/M期比较高于阴性对照组(P<0.05)。干扰YAP组的细胞迁移率低于阴性对照组(P<0.05)。干扰YAP组细胞的Bcl-2、Cyclin D1、MMP2和MMP9的m RNA含量均低于阴性对照组(P<0.05)。结论膀胱癌组织中YAP基因的表达量升高,靶向干扰YAP基因可能是通过减少Bcl-2、Cyclin D1、MMP2和MMP9的表达来抑制细胞的增殖和迁移。【Objective】To study the expression of YAP gene in bladder cancer and regulatory role of YAP targeted interference on bladder cancer cell.【Methods】Bladder cancer tissues and adjacent normal tissues of bladder carcinoma patients were collected and YAP expression was detected. Bladder carcinoma cell line T40 were cultured and YAP was targeted interfered by si RNA fragment transfection. Then cell viability, cell cycle, migration ability and m RNA contents of related genes were detected. 【Results】The m RNA and protein level of YAP in bladder cancer tissues were higher than those in normal tissues（P〈0.05）. MTT value and G0/G1 phase ratio, S phase ratio of YAP interference group were lower than those of negative control group, while G2/M phase ratio was higher（P〈0.05）. The migration rate of YAP interference group were lower than that of negative control group（P〈0.05）. The m RNA contents of Bcl-2, Cyclin D1, MMP2, MMP9 of YAP interference group were lower than those of negative control group（P〈0.05）. 【Conclusions】Expression of YAP gene in bladder cancer is increased, and targeted interference of YAP can inhibit proliferation and migration of cells by reducing the expression of Bcl-2,Cyclin D1, MMP2, MMP9.

关 键 词：膀胱癌 YAP基因 增殖 迁移 细胞周期 

分 类 号：R737.14[医药卫生—肿瘤]