反义VEGF基因抑制人胶质细胞瘤BT325生长的研究  

Inhibition of antisense VEGF gene on the growth of glioma cells BT325

作　　者：孙丽翠[1] 卢佳希[1] 宫伟彦[1] 刘轶群[1] 韩枫[1] 王琴[1] 张双庆[1] 黄振武[1] 

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《卫生研究》2015年第3期413-416,共4页Journal of Hygiene Research

基　　金：北京市教委基金项目(No.099KJ-08)

摘　　要：目的研究反义VEGF基因对人胶质细胞瘤BT325生长的抑制作用及由此引起的细胞凋亡。方法构建含VEGF反义基因的真核表达载体pc DNA3.1/anti-VEGF(实验组),将其转染人胶质细胞瘤BT325,ELISA检测VEGF的表达。采用软琼脂集落生成,MTT以及电子显微镜观察胶质细胞瘤的增殖及凋亡等变化。结果与对照组相比,实验组VEGF的表达显著降低,几乎完全被抑制。与空载体组细胞集落生成数(21)相比,实验组细胞集落生成数(2)明显减少,胶质细胞瘤的生长受到明显的抑制,抑制率为38.23%,且电镜下实验组细胞出现明显的凋亡形态。结论反义VEGF基因通过抑制VEGF的表达抑制人胶质细胞瘤的增殖,促进细胞凋亡,发挥抗癌作用。Objective To investigate the inhibitory effect of antisense VEGF gene on growth of human glioma BT325 and induced apoptosis in cells. Methods The pcDNA3.1/anti-VEGF eukaryotic expression vector was constructed and transfected into human glioma BT325. The ELISA assay was used to assess the expression of VEGF gene. Soft agar colony formation, MTT assay and electron microscope were used to evaluate the proliferation, apoptosis and the morphological changes of BT325. Results Compared with a control group, the level of VEGF expression was significantly decreased and was almost completely suppressed. The amount in soft agar colony at vector group and antisense VEGF gene group were 21 and 2, respectively. The growth of BT325 was significantly inhibited to 38.23% and resulted in the apoptotic morphology by antisense VEGF gene under electron microscope, compared to vector group. Conclusion Antisense VEGF gene inhibited the growth and proliferation, induced cell apoptosis, played an efficient role of anti-cancer in BT325 cells.

关 键 词：反义VEGF 人胶质细胞瘤 ELISA MTT 细胞凋亡 

分 类 号：R730.264[医药卫生—肿瘤] R730.1[医药卫生—临床医学]