EB病毒抗体及其DNA联合检测在鼻咽癌筛查和早期诊断中的价值  

Combined determination of Epstein-Barr virus-related antibodies and EBV DNA for nasopharyngeal carcinoma early diagnosis

作　　者：邱厚匡[1] 姚亚超[1] 李磊[2] 李亚红[1] 张珍[1] 李楠[1] 严芳[1] 李泽泳[1] 吴春燕[1] 吴晓昀[1] 张智[1] 

机构地区：[1]广东省第二人民医院中心实验室,广州510317 [2]广州医科大学附属第三医院生殖医学中心/广东省生殖医学重点实验室,广州510150

出　　处：《检验医学与临床》2015年第10期1339-1341,1344,共4页Laboratory Medicine and Clinic

基　　金：国家自然科学基金项目(81400639);广州医科大学博士启动基金(2014C39)

摘　　要：目的探讨EB病毒(EBV)抗体及EBV DNA联合检测在鼻咽癌筛查和早期诊断中的价值。方法收集2011年7月至2014年8月广东省第二人民医院经病理确诊的鼻咽癌患者272例,采用酶联免疫法(ELISA)测定血清RTA-IgG、VCA-IgA和EA-IgA;荧光定量聚合酶链反应(PCR)测定血浆EBV-DNA。结果四项指标联合检测时,其敏感度最高(99.63%);早期鼻咽癌的阳性率为97.62%(41/42),晚期鼻咽癌的阳性率为100.00%(230/230)。四项指标中任意两项及两项以上指标为阳性的特异性在93.00%以上。结论四项指标联合检测可用于早期鼻咽癌的筛查,任意两项以上指标阳性时应高度怀疑鼻咽癌。Objective To evaluate the values of combined detection of Epstein-Barr virus-related antibodies and EBV DNA for diagnosing of early stage nasopharyngeal carcinoma（NPC）.Methods A total of 272 NPC patients were recruited in this study from July 2011 to August 2014 in the Second People′s Hospital of Guangdong Province.Euzymelinked immunosorbent assay was performed to detect RTA-IgG,VCA-IgA and EA-IgA antibody,and realtime fluorescent quantitative PCR for measuring EBV DNA in plasma.Results The sensitivity of the combined detection for diagnosing NPC was 99.63% .Combined detection could improve the positive rate of diagnosis for early and late stage NPC to 97.62% （41/42）and 100.00% （230/230）respectively.The positive rate of any two or more indicators for NPC was above 93.00% .Conclusion Combined determination could be helpful to screen the early stage NPC,when people has any two or more positive indicators should be suspected to have NPC.

关 键 词：鼻咽癌 EB病毒抗体 EBV DNA 早期诊断 

分 类 号：R739.63[医药卫生—肿瘤]