肝细胞癌患者肿瘤循环DNA的变异检测及其临床意义  

Mutations detection of circulating tumor DNA in patients with hepatocellular carcinoma and its clinical significance

作　　者：逄锦忠 钦伦秀 汤钊猷[2] 

机构地区：[1]青岛经济技术开发区第一人民医院普外二科,青岛266555 [2]复旦大学肝癌研究所、中山医院肝外科,上海200032

出　　处：《国际外科学杂志》2015年第4期257-260,共4页International Journal of Surgery

基　　金：国家杰出青年科学基金（No.30325041）;国家科技攻关计划（No.2002BA711A02-4）

摘　　要：肿瘤循环DNA的变异检测可分为定量和定性两种：前者主要检测循环DNA（血清或血浆）的总量,后者主要检测血清或血浆中肿瘤特异性基因的改变,如基因突变、抑癌基因的甲基化及微卫星变异等.两者均可反映肿瘤的存在和严重程度.本文现就肝细胞癌患者肿瘤循环DNA的变异检测及有关临床意义作一简要综述.Mutations detection of circulating tumor DNA can be divided into quantitative and qualitative classifications：the forumer mainly detects the total amount of circulating DNA （serum or plasma）,whereas the latter mainly detects the specific genetic variations in serum or plasma DNA,such as gene mutations,methylations of tumor suppressor genes,and microsatellite alterations,etc.Both of them may reflect the tumor presence and disease severity.In this paper,mutations detection and its clinical significance of circulating tumor DNA in patients with hepatocellular carcinoma are reviewed.

关 键 词：癌 肝细胞 循环DNA 基因组结构变异 

分 类 号：R730.54[医药卫生—肿瘤]