胃癌组织中亲环素A表达与肿瘤细胞体外化疗药物敏感性的关系  

Relationship between cyclophilin A expression and chemosensitivities of gastric carcinoma cells in vitro

作　　者：王冬[1] 李勇[1] 赵群[1] 范立侨[1] 檀碧波[1] 

机构地区：[1]河北医科大学第四医院外三科,河北石家庄050011

出　　处：《临床荟萃》2015年第5期525-528,共4页Clinical Focus

摘　　要：目的分析胃癌组织中亲环素A(CypA)表达与肿瘤细胞体外化疗药物敏感性及耐药相关蛋白的关系,并探讨其意义。方法磺酰罗丹明B(Sulforhodamine B,SRB)法检测94例胃癌组织对氟尿嘧啶(5-FU)、奥沙利铂(L-OHP)、阿霉素(ADR)的体外化疗药物敏感性;对相应石蜡标本进行免疫组化染色,检测CypA蛋白及耐药相关蛋白P-糖蛋白(P-glycoprotein,P-gp)、谷胱甘肽-S-转移酶-π(Glutathione S-transferase-π,GST-π)、x-IAP、Survivin的表达。结果 CypA、P-gp、GST-π、x-IAP、Survivin阳性表达率分别为72.3%(68/94)、90.4%(85/94)、62.8%(59/94)、66.0%(62/94)、76.6%(72/94),均明显高于癌旁组织(均P<0.05)。5-FU、L-OHP对CypA强表达的肿瘤细胞抑制率低于弱表达者(均P<0.05),而CypA的表达情况与ADR对肿瘤细胞的抑制率无明显关系(P>0.05)。相关分析显示,CypA蛋白与P-gp、x-IAP、Survivin均存在正相关关系(均P<0.05)。结论胃癌细胞对5-FU、L-OHP药物敏感性与CypA蛋白表达有关,该作用可能是CypA调节部分耐药相关蛋白实现的。Objective To analyze the relationship between chemosensitivities of gastric cancer cell,drug-resistant related protein and protein expression of cyclophilin A （CypA）in vitro,and to explore its significance. Methods In 94 tissues of gastric cancer,the chemosensitivities of tumor cell to 5-FU,L-OHP,ADR in vitro were tested by SRB assay, and expression of CypA, P-gp, GST-π, x-IAP and survivin were determined by immunohistochemical method.Results The positive expression of CypA,P-gp,GST-π,x-IAP,survivin were 72.3%（68/94）,90.4%（85/94）,62.8%（59/94）,66.0%（62/94）and 76.6%（72/94）,respectively,and protein expression of CypA,P-gp,GST-π,x-IAP,survivin were significantly higher in tumor cell than in adjacent non-tumor cell （P 0.05）.CypA protein was positively associated with P-gp,x-IAP and survivin （P 〈0.05）.Conclusion The protein expression of CypA was associated with the chemosensitivities of 5-FU, L-OHP in gastric cancer cell,which may be implemented by CypA through regulating drug-resistant related proteins.

关 键 词：胃肿瘤 亲环素 A 抗药性 肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]