生物钟基因Cry1在消化道腺癌中的表达及其与临床病理特征的关系  

Expression of circadian clock gene cryptochrome-1 in gasrointestinal adenocarcinoma and its relationship with clinicopathologic characteristics

作　　者：刘海军[1] 徐蕾[1] 凌烈峰[1] 王文军[2] 何雷[2] 冯遵永[1] 章尧[1] 

机构地区：[1]皖南医学院生物化学与分子生物学教研室,安徽芜湖241002 [2]皖南医学院附属弋矶山医院病理科,安徽芜湖241001

出　　处：《皖南医学院学报》2015年第3期205-208,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金青年项目(30900243)

摘　　要：目的:探讨生物钟基因Cry1在消化道腺癌中的表达及其与临床病理特征之间的关系。方法:采用免疫组织化学法检测生物钟基因Cry1在63例消化道腺癌组织和对应癌旁组织中的表达,分析两者之间的关系以及Cry1的表达与临床病理特征之间的关系。结果:生物钟基因Cry1在63例消化道腺癌组织和对应癌旁组织中表达强度无显著相关性(P>0.05),Cry1的高表达与肿瘤的低分化相关(P<0.05);Cry1的表达定位在细胞核和细胞质中,癌组织和癌旁组织之间的亚细胞分布差异具有显著统计学意义(P<0.01)。结论:生物钟基因Cry1在低分化腺癌中的表达增高以及在癌组织中的亚细胞定位发生改变,提示Cry1蛋白定位在消化道腺癌的发生发展中可能发挥作用,Cry1的表达也可能与肿瘤的恶性程度有关。Objective： To investigate the expression of circadian clock gene cryptochrome-1（ Cry1） in gastrointestinal adenocarcinoma and its relationship with clinicopathologic characteristics. Methods： Immunohistochemistry was used to detect the expression of Cry1 in 63 clinical specimens from tumor and its adjacent tissues. Statistical analysis was performed to evaluate the relationship between tumor tissue and its adjacent tissues as well as association of Cry1 expression with clinicopathologic paramenters. Results： Cry1 expression was not significant in cancerous and noncancerous tissues from 63 patients of gastrointestinal adenocarcinoma（ P 〉0. 05）,yet upregulated Cry1 expression indicated poorly differentiated gastrointestinal adenocarcinoma（ P〈0. 05）. Cry1 was stained in the cytoplasm and nuclear regions,which suggested statistical difference of subcellular distribution between carcinoma and adjacent mucosa.（ P〈 0. 01）. Conclusion： Upregulated expression of Cry1 in poorly differentiated adenocarcinoma and the change of subcellular distribution in cancer tissues indicate that subcellular localization of Cry1 may play a role in the development of gastrointestinal adenocarcinoma,and the expression level of this protein may affect the malignancy of tumor.

关 键 词：生物钟基因 CRY1 消化道腺癌 免疫组织化学 

分 类 号：R735[医药卫生—肿瘤]