复合载体固定化果胶酶澄清枇杷果汁的工艺优化  

Optimization of clarification technology for loquat juice in pectinase immobilized on composite support

作　　者：林建城[1] 张雄[1] 

机构地区：[1]莆田学院环境与生物工程学院,莆田351100

出　　处：《食品科技》2015年第5期49-54,共6页Food Science and Technology

基　　金：福建省高校服务海西重点建设项目(2008HX02);福建省教育厅资助省属高校科研基金项目(JK2012046)

摘　　要：以戊二醛为交联剂,利用Fe3O4磁核与海藻酸钠和明胶制备成磁性复合载体,以复合载体固定化果胶酶,采用正交试验对复合载体固定化果胶酶澄清"解放钟"枇杷果汁的工艺进行优化。结果表明:固定化果胶酶澄清枇杷果汁的技术参数为:固定化酶用量35 g/L(果汁),果汁p H值3.0,在50%果汁浓度中50℃下反应90 min,此条件下,固定化酶处理后果汁的透光率比原汁透光率提高了70%以上;固定化果胶酶重复使用10次后,枇杷果汁透光率还可达到50.3%。在连续搅拌罐式固定化果胶酶反应器中,枇杷果汁以1.8 m L/min流速和60 r/min搅拌速度为适宜参数,酶可反复连续使用,果汁澄清效果好。固定化果胶酶处理后果汁的糖酸度略有下降,果渣汁分层速度加快,果汁变为淡黄色,冷热稳定性得到提高。The composite support was prepared with Fe3O4 magnetic nucleus and sodium alginategelatin. Pectinase was immobilized with composite support which crosslinked with glutaraldehyde. The immobilized pectinase was used to clarify loquat juice. The clarifi cation technology for loquat juice was determined through orthogonal experiments based on single factor. The results showed that the process conditions were as follows： amount of immobilized enzyme given for 35 g/L（juice）, p H value of juice for 3.0, concentration of juice for 50%, and 50 ℃ for 90 minutes. Under this condition, light transmittance of juice could improve over 70% compared with the untreated group. Light transmittance of loquat juice could reach to 50.3% after immobilized pectinase was repeatedly used ten times. The technical parameters for clarifying loquat juice in continuous stirred tank reactor were： fl ow rate of 1.8 m L/min for juice and stir speed of 60 r/min. Immobilized enzyme could be repeatedly used and the clarifi cation effect was better. Compared with the untreated group, the sugar content and titratable acidity of juice had a slight decrease. The separation between juice and sediment became easier. The color of loquat juice became thin yellow, and showed higher stability to cold and heat.

关 键 词：复合载体 固定化果胶酶 枇杷果汁 透光率 连续搅拌罐式酶反应器 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]