HPLC法测定维吾尔药大花罗布麻叶中异槲皮苷的含量  

Determination of isoquercitrin in leaves of Pocvnum hendersonii( Hook. f. )Woodson of Uyghur crude drug by HPLC

作　　者：穆建 佟瑞敏 苏来曼.哈力克 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]新疆维吾尔自治区食品药品检验所,乌鲁木齐830004

出　　处：《新疆医科大学学报》2015年第6期718-720,共3页Journal of Xinjiang Medical University

基　　金：国家药典委员会<中国药典2015年版>科研基金(2013-M43)

摘　　要：目的建立高效液相色谱法测定大花罗布麻叶中异槲皮苷含量的方法。方法采用高效液相色谱法对样品中异槲皮苷进行定量分析,以十八烷基硅烷键合硅胶为填充剂;甲醇-乙腈-0.3%磷酸（11∶10∶79）为流动相,柱温为35℃,检测波长为354 nm。结果异槲皮苷进样量在浓度为0.020 2~0.202 0 mg/m L（r=0.999 9）范围内与其峰面积呈良好线性关系。平均回收率为104.45%（n=6）,RSD为0.45%。10批不同产地样品中异槲皮苷的含量为0.320%~0.392%,平均含量为0.37%。结论 HPLC色谱法对大花罗布麻叶中异槲皮苷进行测定,方法简便,精确,重现性好,为有效制订质量标准提供科学依据。Objective To establish a method for the determination of isoquercitrin in leaves of Pocvnum hendersonii （Hook. f.） Woodson by HPLC. Methods HPLC method was adopted for a quantitative analysis of isoquercitrin. It was carried out on C18 column, with the mobile phase of methnal-acetonitrile --0.3% phosphoric acid water （11： 10 ： 79）, the flow rate of 1.00 ml/min, the column temperature of 35℃ and the wavelength for detection of 354 nm. Results There were good linear relationships between the peak area and concentration at the range of 0.020 2~0.202 mg/mL for isoquercitrin （r =0.999 9）. The average recovery rate of isoquercitrin was 104.45% （n =6）, with RSD of 0.45%. The average content of ten different samples was 0.37%. Conclusion HPLC method to determine the isoquercitrin in leaves of Pocvnum hendersonii （Hook.f.） Woodson was proved to be simple, accurate and reproducible, which can provide scientific basis for quality control of leaves of Pocvnum hendersonii （Hook.f.） Woodson.

关 键 词：维吾尔药 大花罗布麻叶 异槲皮苷 高效液相色谱法 

分 类 号：R914[医药卫生—药物化学]