慢性髓系白血病中let-7a-3甲基化态势及其临床意义  

Methylation situation of let-7a-3 in chronic myeloid leukemia and its clinical significance

作　　者：吴得红 姚冬明[3] 李云[1] 林江[4] 邓兆群[4] 杨静[1] 陈星星[1] 钱震[1] 马吉春[4] 钱军[1] 

机构地区：[1]江苏大学附属人民医院血液科,江苏镇江212002 [2]江苏省昆山市第三人民医院血液科,215300 [3]江苏大学附属人民医院检验科,江苏镇江212002 [4]江苏大学附属人民医院中心实验室,江苏镇江212002

出　　处：《重庆医学》2015年第15期2020-2023,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81270630;81172592;SH2013042);镇江市社会发展项目(SH2010015;SH2011056);江苏省"333工程"科研项目资助计划(BRA2011085;BRA2013136);江苏省科技厅临床医学科技专项资金项目(BL2012056);镇江市科技基础设施建设项目(SS2012003)

摘　　要：目的探讨慢性髓系白血病(CML)患者中let-7a-3启动子的甲基化态势及其临床意义。方法建立实时定量甲基化特异性PCR(RQ-MSP)分别检测25例对照者及52例CML患者骨髓单个核细胞中let-7a-3启动子未甲基化水平。结果 52例CML患者未甲基化let-7a-3启动子(59.6%)为阳性,而对照组仅1例(4%)阳性,两组比较差异有统计学意义(P<0.01)。ROC曲线分析表明,let-7a-3启动子未甲基化作为辅助诊断CML有较好的特异性。未甲基化let-7a-3启动子水平与BCR/ABL融合基因转录水平呈显著正相关(r=0.641,P=0.001),但与患者的白细胞、血小板计数及血红蛋白水平无明显相关性(P>0.05)。在慢性期和加速期let-7a-3未甲基化水平显著高于急变期。结论 let-7a-3基因低甲基化水平随疾病进展而降低。Objective To investigate the methylation situation of let‐7a‐3 promoter in patients with chronic myeloid leukemia （CML） and its clinical significance .Methods The methylation level of let‐7a‐3 promoter in the bone marrow mononuclear cells of 52 CML patients and 25 controls was detected by using the real‐time quantitative methylation‐specific PCR （RQ‐PCR） .Results The non-hypomethylation of let‐7a‐3 promoter was positive in 31 cases（59 .6% ） of 52 CML patients ,while only 1 case（4% ,1/25） in the control group ,the difference between the two groups were statistically significant （P〈 0 .01） .The ROC curve analysis showed that the non -hypomethylation of let‐7a‐3 has better specificity for the auxiliary diagnosis of CML .The significantly posi‐tive correlation was found between the non -methylation level of let‐7a‐3 promoter and the BCR/ABL transcription level （r=0 .641 ,P=0 .001） .In contrast ,there was no obvious correlation between the non -methylation level of let‐7a‐3 promoter and the WBC count ,platelet count and hemoglobin levels（P〉0 .05） .The non-hypomethylation level of let‐7a‐3 in chronic phase and accel‐erate phase was significantly higher than that in blastic crisis of CML .Conclusion The hypomethylation level of let‐7a‐3 promoter is decreased with disease progression .

关 键 词：慢性髓系白血病 let-7a-3 低甲基化 微小RNA 

分 类 号：R733.7[医药卫生—肿瘤]