神经生长因子对四氯化碳诱导的小鼠肝纤维化的影响  

Effect of nerve growth factor on CCL_4-induced hepatic fibrosis in mice

作　　者：张伟[1] 刘翠芸[2] 刘佩佩[2] 付文娟[2] 孙水林[2] 席文娜[2] 

机构地区：[1]南昌大学附属感染病医院肝病科,南昌330000 [2]南昌大学第二附属医院感染性疾病科,南昌330006

出　　处：《重庆医学》2015年第15期2024-2027,共4页Chongqing medicine

基　　金：江西省卫生厅基金资助项目(20071077)

摘　　要：目的观察神经生长因子(NGF)对四氯化碳(CCL4)诱导的小鼠肝纤维化的影响。方法将30只雌性昆明小鼠分成3组:纤维化模型组(A组)、NGF干预组(B组)和生理盐水对照组(C组)。8周时采集标本,用全自动生化分析仪检测丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、清蛋白(ALB);放射免疫法检测肝纤维化指标:透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)。采用Ishaki评分系统对小鼠肝组织炎症活动度及纤维化程度进行评分。结果A、B组ALT、AST、HA、LN均明显高于C组(F=111.45,658.80,157.43,167.99;P<0.05);B组ALT、AST、LN均明显低于A组(P<0.05)。HE染色、网状纤维染色及Masson染色显示,A组肝组织炎症及纤维化程度最明显,B组肝组织炎症较A组显著减轻,未形成纤维间隔,纤维组织细短,C组肝组织未见明显炎症细胞浸润及纤维形成。A组肝脏炎症分级和纤维化分期评分较B、C组高,且3组炎症活动度得分、纤维化得分差异有统计学意义(均P<0.05)。结论 NGF可以阻断CCL4诱导的小鼠肝纤维化,减轻肝组织炎症。Objective To observe the effect of nerve growth factor（NGF） on CCL4‐induced hepatic fibrosis in mice .Methods The hepatic fibrosis model was induced by subcutaneous injection of CCL 4 in mice .Thirty female Kunming mice were equally and randomly divided into three groups ：fibrosis model group （A） ,NGF intervention group （B） and normal saline control group （C） .At 8 weeks following the initiation of experiment ,the samples were collected to measure ALT ,AST ,TBIL ,ALB by the fully automativ biochemical analyzer ,an the liver fibrosis indices （HA ,LN ,PC Ⅲ ） by radioimmunoassay .The Ishaki scoring system was adopted to assess the severity of hepatic inflammation and fibrosis degree .Results Serum levels of ALT ,AST ,HA and LN in the group A and B were significantly higher than those in the group C （F= 111 .45 ,658 .80 ,157 .43 ,167 .99 ;P〈 0 .05） ,the levels of ALT 、AST and LN in the group B were significantly lower than those in the group A （P〈 0 .05） .The HE staining ,reticular fiber staining and Masson staining showed that the liver fibrosis degree and the liver tissue inflammation in the group A were most obvious ,the liver tissue inflamation in the group B were significantly alleviated as compared with the group A .No fibrous septum was formed and the fiber tissues were fine and short .No obvious inflammatory cells infiltration and fibers formation were found in the liver tissue of the group C .The scores of liver inflamation grade and fibrosis staging in the group C were higher than those in the group B and C ,moreover the scores of liver inflammation grade and fibsosis had statstical differences among 3 groups （P 〈 0 .05） .Conclusion NGF can block hepatic fibrosis induced by CCL4 and relieve the liver inflammation .

关 键 词：神经生长因子 四氯化碳 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]