Treg细胞介导的免疫应答在牙周炎小鼠中的研究  

The study on Treg cell-mediated immune response in mice with periodontitis

作　　者：王琳源[1] 关宁[2] 林晓萍[3] 

机构地区：[1]辽宁医学院附属第二医院牙周科,锦州121000 [2]辽宁医学院附属第一医院脑与脊髓损伤重点实验室 [3]中国医科大学附属盛京医院口腔科

出　　处：《实用口腔医学杂志》2015年第3期318-322,共5页Journal of Practical Stomatology

基　　金：辽宁省科学技术计划项目(编号:2012225015);高等学校博士学科点专项科研基金(编号:20112104110013)

摘　　要：目的:研究牙周炎小鼠中调节性T(Treg)细胞介导的免疫应答情况。方法:将8只7周龄C57BL/6雌性小鼠随机分为2组(n=4)。通过口腔涂抹牙龈卟啉单胞菌(P.gingivalis)建立牙周炎模型,对照组口腔涂抹羧甲基纤维素。涂抹结束后第4周处死小鼠,流式细胞仪检测CD4+叉头翼螺旋转录分子(Foxp3)+细胞;ELISA检测转化生长因子(TGF)-β1和白细胞介素(IL)-10的表达。结果:牙周炎小鼠牙龈、颈部淋巴结和外周血中CD4+Foxp3+细胞在CD4+T细胞中的比例降低(P<0.01)。牙周炎小鼠TGF-β1在牙龈组织中的表达以及IL-10在牙龈、颈部淋巴结和血清中的表达增高(P<0.05)。结论:在牙周炎的发病过程中,Treg细胞介导的免疫应答减弱,牙龈、颈部淋巴结和外周血可能是牙周炎中Treg细胞主要的细胞来源。Objective：To investigate regulatory T（Treg）cell-mediated immune response in mice with periodontitis.Methods：8 sev-en-week old C57BL/6 female mice were randomly divided into 2 groups（n =4）.Periodontitis was induced in mice by oral infection with Porphyromonas gingivalis（P.gingivalis）.Carboxymethyl cellulose（CMC）was used for the sham control mice.All mice were sacrificed 4 weeks after the last P.gingivalis infection.CD4 ＋forkhead box P3 （Foxp3）＋ cells were analyzed by flow cytometry.The protein ex-pression of TGF-1 and IL-1 0 was detected by ELISA.Results：Periodontitis mice showed an obvious decrease in the percentage of CD4 ＋Foxp3 ＋ cells in CD4 ＋ T cells in gingival tissues,cervical lymph nodes（CLNs）and peripheral blood（P 〈0.01 ）.Moreover,peri-odontitis mice had an obvious increase in the expression of TGF-1 and IL-1 0 in gingival tissues,CLNs and serum（P〈0.05）.Conclu-sion：Treg cell-mediated immune response is weakened in the mice with periodontitis.Gingival tissues,CLNs and the peripheral blood are probably the main source of Treg cells during periodontitis.

关 键 词：调节性 T 细胞 牙周炎 骨吸收 

分 类 号：R781.42[医药卫生—口腔医学]