不同表面特性种植体对成骨细胞增殖及IL-6、Cbfα1 mRNA表达的影响  

Titanium specimens with different surface character affect the proliferation and IL-6 and Cbfα1 expression in osteoblasts

作　　者：薛鹏飞[1] 侯玉东[1] 潘晶晶[2] 孙龙[1] 孙鑫[1] 

机构地区：[1]滨州医学院口腔医学院,烟台264003 [2]潍坊医学院医学影像学系

出　　处：《实用口腔医学杂志》2015年第3期335-338,共4页Journal of Practical Stomatology

摘　　要：目的:探讨不同表面特性种植体对成骨细胞增殖及IL-6、Cbfα1 mRNA表达的影响。方法:用机械磨光(PT)、喷砂酸蚀(SLA)、阳极氧化(AD)法制备3种不同表面的纯钛标准试件,SEM观察表面形貌并分析表面元素构成;接触角测量仪检测各组表面静态水接触角;将成骨细胞(MC3T3-E1 Sub-clone 14)培养于试件表面,细胞培养板为对照,MTT法检测接种1、3、5、7 d细胞增殖率,RT-q PCR法检测接种6、12、24 h白细胞介素6(IL-6)和核心结合因子(Cbfα1)mRNA表达。结果:PT组表面呈机械划痕,SLA组呈大小、深浅不一的窝洞,AD组呈较均匀的圆孔状,3组表面主要元素分别为Ti、Ti/Al、Ti/O。3组表面静态水接触角分别为54.47°±3.33°、75.42°±8.32°、38.91°±4.00°(P<0.05);MTT显示各实验组细胞增殖率均低于对照组(P<0.05),且AD>PT>SLA(P<0.05);RT-q PCR显示在同一时间点各实验组IL-6 mRNA表达下调、Cbfα1 mRNA的表达上调(P<0.05),且AD>SLA>PT(P<0.05)。结论:AD相对PT及SLA表面能促进成骨细胞增殖、下调IL-6 mRNA表达及上调Cbfα1 mRNA表达,提示AD种植体表面在种植早期可获得更好更快的骨结合。Objective：To investigate the effects of titanium spcimens with different surface character on the proliferation and mRNA expression of IL-6 and Cbfα1 in osteoblasts.Methods：Titanium surface was treated by smooth pretreatment（PT）,sandblast and acid etch（SLA）and anodic oxidation（AD）respectively.The morphology and the elements analysis of the spcimens were inspected and detected by SEMand EDS.The surface contact angle was measured by contact angle meter.MC3T3-E1 cells were cultured on the titanium surface and cells cultured on tissue culture plate were served as the control group.The proliferation was measured by MTT assay.The mRNA expression of IL-6 and Cbfα1 was quantified by RT-qPCR.Results：The sample surface in PT group showed scrat-ches,in SLA group showed multiple three dimensional structure,in AD group exhibited porous structure.The elements of the sample surface of group PT,SLA and AD were Ti,Ti/Al and Ti/O respectively;the contact angles were 54.47°±3.33°;,75.42°±8.32°; and 38.91°±4.00°;respectively（P〈0.05）.The cells in AD group showed higher proliferation than those in PT and SLA groups（P〈0.05）.In AD group IL-6 mRNA expression decreased and Cbfα1 mRNA increased more than in PT and SLA groups（P〈0.05）. Conclusion：Titanium spcimens treated with AD may promote cell proliferation,decrease IL-6 mRNA expression and increase Cbfα1 mRNA expression in MC3T3 cells.Implats treated with AD might have some advantages in early osseointegration.

关 键 词：表面特性 喷砂酸蚀 阳极氧化 成骨细胞 

分 类 号：R783[医药卫生—口腔医学]