负载PTH(1-34)聚乳酸微球体外促成骨分化作用的研究  

PLGA microsphere loading with PTH( 1-34) promotes osteogenesis: An in vitro study

作　　者：王晶晶[1] 孟维艳[1] 张喆[2] 周延民[1] 张士博[1] 蔡青[1] 刘童斌[1] 袁月[1] 

机构地区：[1]吉林大学口腔医学院种植中心,长春130021 [2]中国科学院长春应用化学研究所高分子物理与化学国家重点实验室

出　　处：《实用口腔医学杂志》2015年第3期360-364,共5页Journal of Practical Stomatology

基　　金：吉林省产业技术研究与开发专项项目(编号:2013C022-6)

摘　　要：目的:评价包载PTH(1-34)聚乳酸微球的缓释效应对前成骨细胞MC3T3-E1成骨分化的影响。方法:将MC3T3-E1细胞分为空白培养液对照组、持续性和间歇性给药组、未载药PLGA微球组和载药PLGA微球组。通过碱性磷酸酶(ALP)染色、茜素红染色和Reverse Transcription PCR观察负载PTH(1-34)的PLGA微球对细胞成骨分化的影响。结果:释放终浓度为10-9mol/L的载药PLGA微球组可以提高成骨细胞ALP活性,促进矿化,上调RUNX2、ALP和VEGF基因的表达。结论:PLGA微球缓释PTH(1-34)可促进成骨细胞分化。Objective：To determin the effect of PLGA microspheres loading with PTH（1-34）[PTH（1 34）/PLGA]on the differentiation of MC3T3E1 cells.Methods：MC3T3E1 cells were divided into control group,continuous or intermittent PTH（1-34）adminstration groups,PLGA microsphere group and PTH（1-34）/PLGA group.Osteogenesis differentiation was observed by alkaline phosphatase activity（ALP）,alizarin red staining and RTPCR.Results：The PTH（1 34）/PLGA with 10-9 mol/L final release concentration enhanced ALP activity and mineralization,increased the mRNA expression of RUNX2,ALP and VEGF.Conclusion：Controlledrelease of PTH（1-34）from PLGA microspheres can promote the osteogenesis differentiation of MC3T3E1 cells.

关 键 词：甲状旁腺素1-34(PTH(1-34)) PLGA 缓释 

分 类 号：R783.1[医药卫生—口腔医学]