完善根管充填后治疗失败感染根管中粪肠球菌的活菌研究  

Quantitative evaluation of live Enterococcus faecalis in failed endodontic root-well-filled teeth

作　　者：张富华[1] 张建凤[1] 何爱民[1] 韦智君 

机构地区：[1]合肥市口腔医院牙体牙髓二科,230001

出　　处：《实用口腔医学杂志》2015年第3期365-368,共4页Journal of Practical Stomatology

基　　金：安徽省年度重点科研项目(编号:11070403040)

摘　　要：目的:将叠氮溴化丙锭(PMA)与定量PCR(q PCR)技术结合,用于计算根管治疗失败感染根管中粪肠球菌活菌数量。方法:选取34名根管治疗失败患者,提取感染根管内细菌DNA,每个样本分成2份,一份经PMA处理(实验组),一份未用PMA处理(对照组),然后通过q PCR方法检测样本中粪肠球菌的活菌数量和细菌总量。结果:20例患者(58.8%)细菌样本中检测出粪肠球菌。实验组和对照组Ct值分别为25.12±2.04和24.62±2.02(P=0.001)。结论:对于根管治疗失败感染根管内活菌的研究使用PMA结合定量PCR可能是一种有效的方法。Objective：To analyse viable Enterococcus faecalis（E.faecalis）in root-well-filled teeth associated with failed endodontic treatment by using propidium monoazide （PMA）in combination with real time qPCR.Methods：Bacterial samples were extracted from 34 root-canal-treated teeth with post-treatment apical periodontitis.Each sample was separated into 2 different tubes.PMA was added to one of the tubes,and the other was left untreated.Then,DNA extraction and qPCR were performed.Results：E.faecalis was found in 20 of the 34 samples（58.8%）.In PMA treated and none-treaten samples the Ct value of E.faecalis was 25.1 2 ±2.04 and 24.62±2.02 respectively（P =0.001 ）.Conclusion：PMA may be feasible in differentiating viable and dead Enterococcus faecalis cells.

关 键 词：叠氮溴化丙锭(PMA) 根管感染 粪肠球菌(E.faecalis) 

分 类 号：R781.05[医药卫生—口腔医学]