下颌髁突软骨与股骨头软骨体外生长发育的比较研究  

Comparison of the development of mandibular condylar cartilage and femoral head cartilage in vitro

作　　者：蔺栋鹏 田海锁 赵天一 吕瑾茹 马秦 

机构地区：[1]第四军医大学口腔医院口腔颌面外科.军事口腔医学国家重点实验室,西安710032

出　　处：《实用口腔医学杂志》2015年第3期369-373,共5页Journal of Practical Stomatology

基　　金：十二五军队重点课题(编号:BWS11J046)

摘　　要：目的:比较在体外培养下下颌骨髁突软骨与股骨头软骨的生长发育状况。方法:取新生小鼠的髁突软骨和股骨头软骨进行组织培养,在培养前和培养6周后进行大体观察、HE染色、茜素红染色以及PCNA免疫组化研究。结果:大体观察可以看到髁突软骨培养后,软骨内发生了异常改变,而软骨面积几乎没有变化(P>0.05);HE染色显示部分软骨层结构消失,茜素红染色证实了软骨基质发生了钙化。股骨头软骨培养后,软骨内尚未观察到异常,而软骨面积增大明显(P<0.05);HE染色显示肥大细胞层增厚,茜素红染色显示股骨头软骨基质尚未出现钙化。PCNA免疫组化研究均提示两类软骨在培养后依然具有增殖能力。结论:在体外培养下,髁突软骨基质可以自发钙化。Objective：To study the difference of the development between mandibular condylar cartilage and femoral head cartilage in vitro.Methods：Mandibular condyles and femoral heads were sampled from 1 2 neonatal mice and cultured in vitro.The samples before culture and after 6-week culture were examined by gross observation,HE staining,Alizarin Red staining and PCNA immunohistochem-istry respectively.Results：After in vitro culture,abnormal changes were observed in condyle cartilage,but the surface area of condyle cartilage was not changed（P 〉0.05）.HE staining showed partial cartilage layer structure disappearance and the Alizarin Red staining confirmed calcification in the cartilage matrix.However,calcification was not found in femoral head cartilage,and the surface area of femoral head cartilage increased（P 〈0.05）.HE staining showed the hypertrophied layer was thicker after culture than before and the Alizarin Red staining showed there was no calcification in the femoral cartilage matrix.The immunohistochemistry displayed PCNA posi-tive expression in both cartilage after culture.Conclusion：In vitro,the mandibular condylar cartilage matrix can be spontaneously cal-cificated.

关 键 词：软骨 下颌骨髁突 股骨头 组织培养 体外 钙化 

分 类 号：R782[医药卫生—口腔医学]