杜氏盐藻与4种海洋微藻间的化感作用  

Allelopathy Between Dunaliella Salina and Four Species of Marine Microalgae

作　　者：潘远健 金刚[1] 代建国[1] 丁莉[1] 张丽君[1] 栾崇林[1] 于化泓[2] 

机构地区：[1]深圳职业技术学院深圳市大规模细胞培养技术和细胞资源库公共技术服务平台,广东深圳518055 [2]南昌大学生命科学与食品工程学院,江西南昌330031

出　　处：《深圳职业技术学院学报》2015年第3期47-53,共7页Journal of Shenzhen Polytechnic

基　　金：深圳市科技项目(JC201005280534A;JCY20130331151022276);广东省自然科学基金资助项目(10151805501000007)

摘　　要：利用交叉培养结合高效液相色谱分析的方法,研究了杜氏盐藻与球等鞭金藻、叉鞭金藻、湛江等鞭金藻和小球藻之间的化感作用,并测定了受试后球等鞭金藻和杜氏盐藻的SOD活力.结果表明:(1)杜氏盐藻与球等鞭金藻、青岛叉鞭金藻、湛江等鞭金藻及小球藻之间存在着显著的化感作用,其中,湛江等鞭金藻、青岛叉鞭金藻胞外滤液对杜氏盐藻的生长有明显的抑制作用,杜氏盐藻胞外滤液明显地抑制小球藻的生长;(2)青岛叉鞭金藻、湛江叉鞭金藻、球等鞭金藻和小球藻胞外滤液萃取物分别至少由6种、8种、6种和6种物质组成;(3)微藻胞外滤液对受试藻SOD酶活性有明显影响.Together with high performance liquid chromatography （HPLC） analysis, the method of cross culture is applied to research the allelopathy among the Dunaliella salina, Isochrysis galbana, Dicrateria inomata, Isochrysis zhangjiangensis and Chlorella vulgaris. Besides, the SOD activity of I. galbana and D. salina is measured. Results indicate that： a significant allelopathy exists among the D. salina, I. galbana, D. inornata, I. zhangjiangensis and C. vulgaris, in which the extracellular filtrate of Isochrysis zhangjiangensis and Dicrateria inornata distinctively inhibits the growth of D. salina; the extracellular filtrate of D. salina inhibits the growth of C. vulgaris. The extracellular filtrate of D. inornata, I. zhangjiangensis, I. galbana and C. vulgaris respectively consists of at least 6, 8, 6, 6 kinds of components; The extracellular filtrate has obvious influence on the SOD enzyme activity of the subjected algae.

关 键 词：微藻 杜氏盐藻 化感作用 

分 类 号：Q178.532[生物学—水生生物学]