新型固载酶生物传感器制备及食品中有机磷农药检测  

Preparation of Novel Immobilized Enzyme Electrochemical Biosensor and Detection of Organophosphorus Pesticide in Food

作　　者：伍周玲 燕冰宇[1] 梁东军[1] 郭明[1] 张新鸽 

机构地区：[1]浙江农林大学理学院,浙江临安311300

出　　处：《中国食品学报》2015年第3期166-174,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(No.20877072);浙江省科技厅重大科技专项(2011C12043)

摘　　要：以微晶纤维素为原料,采用新型水热氧化合成方法,制备固载酶基质材料-双醛纤维素。通过固定化酶化学键联技术制备固定化乙酰胆碱酯酶。利用扫描电镜和红外光谱对产物进行结构表征,固载酶修饰玻碳电极制备ACh E生物传感器。以O,O-二甲基-O-（2,2-二氯乙烯基）磷酸酯为反应物,碘化乙酰硫代胆碱为底物,采用循环伏安和差分脉冲伏安法考察新型传感器对农残的检测性能。结果表明：成功制备了新型固载酶电化学生物传感器,其对DDVP检测的线性范围为0.01-50μg/m L,检出限为6.99 ng/m L,具有很好的重现性和稳定性。本结果可为食品有机磷农药残留的检测提供一种新的分析方法。The dialdehyde cellulose （DAC） which is a carrier material of immobilized enzyme was prepared by tak- ing microcrystalline cellulose （MCC） as raw materials and using a new hydrothermal oxidation synthesis method, and it was used to prepare immobilized acetylcholinesterase according to the immobilized enzyme chemically linking technology. The product structure was characterized by SEM and IR. The immobilized enzyme was modified on glassy carbon elec- trode surface, acetyhhiocholine iodide （ATCh） as the substrate, dimethyl dichloro vinyl phosphate （DDVP） as a repre- sentative, researched the detection capabilities of pesticide residues according to cyclic voltammetry and differential pulse vohammetry. The results showed that the immobilized enzyme electrochemical biosensor was made; the determination of DDVP ranged from 0.01 to 50 μg/mL and the detection limit was 6.99 ng/mL. The results of the analysis may provide a new analysis method for organophosphorus pesticide residues.

关 键 词：双醛纤维素 水热合成 乙酰胆碱酯酶 生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TS207.53[自动化与计算机技术—控制科学与工程]