重组酶聚合酶扩增技术检测转基因水稻中的Cry1Ab/c基因  

Detection of Cry1Ab/c Gene in Genetically Modified Rice by Recombinase Polymerase Amplification

作　　者：邓婷婷[1] 黄文胜[1] 程奇[2] 李新实[1] 陈颖[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]中国农业科学院,北京100081

出　　处：《中国食品学报》2015年第3期187-193,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家质检总局科技计划项目(2014IK083);国家重大科技专项(2014ZX08012-001)

摘　　要：重组酶聚合酶扩增技术(RPA)是利用重组酶和单链结合蛋白在常温下协同实现引物与模板的特异结合,以代替传统PCR热循环中的变性和复性过程的新型恒温体外核酸扩增技术。本研究基于RPA技术建立了转基因水稻Cry1Ab/c基因的检测方法,可在37℃恒温条件下快速检测到转基因水稻中的Cry1Ab/c基因,具有较好的特异性,其绝对及相对检测灵敏度分别达到100个拷贝和0.1%(质量分数),适用于基层实验室及现场快速检测转Cry1Ab/c基因水稻及其制品。As the latest isothermal amplification technology, recombinase polymerase amplification （RPA） had replaced the classical thermal cycling by employing recombinase-primer complexes to scan double-stranded DNA and facilitate strand exchange at cognate sites in room temperature. This article reported a method of screening the Cry1Ab/c gene in genetically modified （GM） rice with using RPA assay. The reactions were specific, rapid and operate at constant low temperature 37 ℃. Furthermore, the results also indicated that the method was sensitive in detecting the Cry1Ab/c gene in transgenic rice, proved to be appropriate on-site or on simply poor-equipped laboratories.

关 键 词：重组酶聚合酶扩增(RPA) 转基因水稻 Cry1Ab/c基因 检测 

分 类 号：S511[农业科学—作物学]