保元煎剂对疲劳大鼠自由基、乳酸、ATP的影响  

Effects of Bao Yuan Decoction on Hydroxyl Radical, Lactic Acid and ATP in Fatigue Rats

作　　者：郭亮锋 董晓静[1] 王建[1] 张红[1] 李锋[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032

出　　处：《现代生物医学进展》2015年第17期3240-3242,3249,共4页Progress in Modern Biomedicine

基　　金：陕西省中医管理局科研课题(zy21)

摘　　要：目的:研究保元煎剂对疲劳大鼠力竭游泳时间以及ATP、羟自由基、乳酸含量的影响。方法:选用60只雄性SD大鼠,随机分为正常组、模型组、西洋参组、保元煎剂低、中、高剂量组。采用力竭游泳加睡眠剥夺建立复合疲劳大鼠模型,均予灌胃给药或蒸馏水(保元煎剂低、中、高剂量依次为1.04、2.08、4.15 g·kg-1·d-1;西洋参组给药量为0.27 g·kg-1·d-1),记录大鼠末次力竭游泳时间,检测疲劳大鼠血清ATP、羟自由基、乳酸含量。结果:与模型组比较,保元煎剂各组大鼠末次力竭游泳时间延长,ATP含量升高,羟自由基及乳酸含量下降,均有统计学意义,P<0.05;与西洋参组比较,保元煎剂高剂量组末次力竭游泳时间延长、乳酸含量降低,保元煎剂高、中、低剂量组ATP升高,保元煎剂高、中剂量组自由基水平下降,均具有统计学意义,P<0.05。结论:保元煎剂可提高疲劳大鼠力竭游泳时间,升高疲劳大鼠ATP含量,降低羟自由基、乳酸含量。Objective： To study the effects of Bao Yuan decoction on exhaustion swimming time, the levels of ATP, hydroxyl radical, and lactic acid in fatigue rats. Methods： 60 Sprague-Dawley（SD） male rats were randomly divided into normal group, model group, American ginseng group, low-dose Bao Yuan decoction group, middle-dose Bao Yuan decoction group and high-dose Bao Yuan decoction group. Exhaustion swimming with sleep deprivation was adopted to establish the compound fatigue rat model. Then the changes of last exhausted swimming time and ATP, hydroxyl radical and lactic acid in serum after drug or distilled water intervention by gavage（low-dose, middle-dose and high-dose Bao Yuan decoction was 1.04, 2.08, 4.15 g·kg^-1·d^-1; American ginseng was 0.27 g·kg^-1·d^-1）. Results： Compared with model group, the last exhausted swimming time was prolonged, the level of ATP was increased, and the level of hydroxyl radical and lactic acid were reduced in Bao Yuan decoction; Compared with American ginseng group, the last exhausted swimming time was prolonged and the content of lactic acid was decreased in high-dose Bao Yuan decoction, the level of ATP was increased in all Bao Yuan decoction, and the level of hydroxyl radical was reduced in middle-dose and high-dose Bao Yuan decoction. All above these were statistically significant（P〈0.05）. Conclusions： Bao Yuan decoction can improve exhaustion swimming time of fatigue rats, increase ATP content and reduce the hydroxyl radicals and lactic acid level in fatigue rats.

关 键 词：保元煎剂 ATP 羟自由基 乳酸 

分 类 号：Q95-3[生物学—动物学] R285.5[医药卫生—中药学]