棘孢曲霉中分离的2个新化合物  

Isolation and Identification of Two New Compounds from Aspergillus Aculeatus

作　　者：徐燕[1] 田沙沙[1] 俞和[1] 杨文智[1] 朱华结[1] 

机构地区：[1]河北大学药物化学与分子诊断重点实验室药学院,保定071002

出　　处：《高等学校化学学报》2015年第6期1107-1111,共5页Chemical Journal of Chinese Universities

摘　　要：从土壤真菌棘孢曲霉(Aspergillus aculeatus)中分离得到2个新化合物和2个已知化合物,采用质谱(MS)、一维核磁共振波谱(1D NMR)、异核多键相关谱(HMBC)、异核单量子相关谱(HSQC)和旋转坐标NOE谱(ROESY)等方法对化合物的结构进行了鉴定,化合物2的顺反构型通过ROESY数据进一步确定.鉴定结果表明,2个新化合物分别为2-(2'-4'-6'-三羟基)-7-羟基-5-甲基色原酮{2-(2',4',6'-trihydroxyphenyl)-(7-hydroxy-5-methyl)chromone}(1)和(E)-4-羟基-3-[(3-甲基-4-羟基-2-丁烯)氧代]苯甲酸{(E)-4-hydroxy-3-[(4-hydroxy-3-methylbut-2-en-1-yl)oxy]benzoic acid}(2);2个已知化合物分别为(S)-2-[(2'-羟基)丙基]-5-甲基-7-羟基色原酮{2-(2'-hydroxypropyl)-5-methyl-7-hydroxychromone}(3)和(3R,4S)-8-羟基-3,4,5-三甲基-6酮-4,6-二氢-3H-异色烯-7-羧酸{(3R,4S)-8-hydroxy-3,4,5-trimethyl-6-oxo-4,6-dihydro-3H-isochromene-7-carboxylic acid}(4).活性测试结果表明,这4个化合物均表现出一定的抗氧化活性.In order to investigate new bioactive compounds, a piece of fungi Aspergillus aculeatus was obtained from the Jinyun mountain soil in Chongqing, China. By fermentation using Potato Dextrose（PD） within 14 d at 28 ℃, the extraction of ethyl acetate（ 103 g） was obtained. The extraction was then used for isolation of compounds through a series of study by classical separation methods, for example Oetadecyl Silane （ODS） and Sephadex LH-20. Two new compounds 2-（2＇, 4＇, 6＇-trihydroxy phenyl） benzene-（7-hydroxy-5-methyl） chromone （ 1 ） and （ E ） -4-hydroxy-3-[ （ 4-hydroxy-3-methylbut-2-en-l-yl ） oxy ] benzoic acid （ 2 ） were obtained. The structures were elucidated using mass spectrometry （MS） , one demensional nuclear magnetic resonance spectroscopy （ 1 D NMR）, heteronuclear multiple bond correlation （ HMQC ）, heteronuclear single quantum correlation （HMBC） and rotating-frame overhauser effect spectroscopy（ROESY）. Other two known compounds 2- （ 2＇-hydroxypropyl ） -5-methyl-7-hydroxychromone （ 3 ） and （ 3R, 4S） -8-hydroxy-3,4,5- trime- thyl-6-oxo-4,6-dihydro-3H-isochromene-7-carboxylic acid （4） were obtained. Compounds 2 to 4 exhibited some 2,2-diphenyl-l-picrylhydrazyl（DPPH） antioxidant activity.

关 键 词：真菌 棘孢曲霉 核磁共振 黄酮 抗氧化活性 

分 类 号：O629.9[理学—有机化学]