产甲烷菌高度紧密子网络分析  

The analysis of highly compact subnetwork of methanogens

作　　者：陈璟[1] 须文波[1] 

机构地区：[1]江南大学物联网工程学院,江苏无锡214122

出　　处：《计算机与应用化学》2015年第5期609-613,共5页Computers and Applied Chemistry

摘　　要：为了从系统水平上阐明嗜热菌的耐热机制,并指导嗜热菌在工业生产上的进一步应用,以产甲烷菌的代谢网络作为研究对象,从研究代谢网络的拓扑结构以及高度紧密子网络出发,探索常温产甲烷菌Methanosarcina acetivorans(M.acetivorans)和嗜热产甲烷菌Methanopyrus kandleri(M.kandleri)之间的代谢网络的耐热性的差异。实验结果发现常温产甲烷菌M.acetivorans最紧密的9-核和嗜热产甲烷菌M.kandleri最紧密的7-核分别包含27和19个酶。其中嗜热产甲烷菌M.kandleri网络中最紧密的7-核被分成2个子网,一个子网中的酶节点刚好是2个产甲烷菌所共同的酶,而另一个子网络中的酶是嗜热产甲烷菌M.kandleri 7-核所特有的酶,这些特有的酶全部与酪氨酸的合成有关。In order to clarify heat-resistant mechanism of these thermophiles from system level and guide further applications of thermophiles in industry, this paper focuses on the research of the metabolic network of methanogens. The paper studied the metabolic network＇s topology structure and highly compact subnetwork, and explored the difference of metabolic network of heat resistance between mesophilie methanogen Methanosarcina acetivorans （M. acetivorans） and thermophilic methanogen Methanopyrus kandleri （M. kandleri）. The experiments shows that the 9-core has 27 enzymes of the mesophilic methanogen M. acetivorans and 7-core of the thermophilic methanogen M. kandleri has 19 enzymes. The 7-core of the thermophie methanogen M. kandleri is divided into two small networks. One has shared enzymes of the two methanogens, the other has particular enzymes of the 7-core of thermophilic methanogen M. kandleri. These unique enzymes are associated with the synthesis of tyrosine.

关 键 词：代谢网络 产甲烷菌 网络拓扑 耐热性 

分 类 号：TQ015.9[化学工程] TP391.9[自动化与计算机技术—计算机应用技术]