过表达嗜热毛壳菌Cu/Zn-SOD基因提高转基因水稻对3种病害的抗性  

Overexpression of Cu/Zn-SOD gene from Chaetom ium therm ophilum enhanced resistance of transgenic rice against three major diseases

作　　者：巨延虎[1] 杨旸[1] 孔令广[1] 李多川[1] 丁新华[1] 储昭辉[1] 

机构地区：[1]作物生物学国家重点实验室/山东省农业微生物重点实验室,山东农业大学植物保护学院,泰安271018

出　　处：《植物病理学报》2015年第3期297-306,共10页Acta Phytopathologica Sinica

基　　金：转基因生物新品种培育科技重大专项(2013ZX08001-002;2013ZX08009-004);高等学校博士学科点专项科研基金(20123702110014);国家自然科学基金资助项目(30900050);山东省泰山学者建设工程专项

摘　　要：超氧化物歧化酶(Superoxide dismutase,SOD,EC1.15.1.1)是生物体中清除氧自由基、保护细胞免受氧化损害的关键保护酶之一,SOD活性的提高可以增加植物对各种不良环境的适应和耐受能力。本研究将一个源自嗜热毛壳菌(Chaetomium thermophilum,Ct)的Cu/Zn-SOD基因Ct SOD通过农杆菌介导的遗传转化方法转入水稻,转基因植株的SOD活性均得到显著提高。对转基因植株分别接种水稻白叶枯病、细菌性条斑病以及纹枯病的病原,发现过量表达Ct SOD能够显著提高转基因植株对3种病害的抗性。这种对多种病害具有抗性的单个基因在植物抗病育种中具有重要应用价值。The superoxide dismutases（ SOD） play critical roles in the defense against oxidative stresses. Many studies have show n that improving SOD activity can increase the adaptation and tolerance to many environmental stresses for plants. Over-expression of SOD genes in plants can enhance their resistance to harmful environmental challenges. In this study,it w as reported that transgenic rice constitutively expressing a Cu / Zn-SOD gene from Chaetomium thermophilum significantly improved their SOD activity and resistance to three major rice diseases,including bacterial blight,bacterial leaf streak and rice sheath blight. This single gene conferring resistance to various diseases could be important in the resistant breeding in plant.

关 键 词：超氧化物歧化酶 细菌性条斑病 白叶枯病 纹枯病 

分 类 号：S432.1[农业科学—植物病理学]