南药植物高良姜内生细菌多样性及其促生潜力  

Diversity and plant growth-promoting potential of bacterial endophytes of Alpinia Officinarum Hance,a famous south-China medicinal plant

作　　者：李淑彬[1] 黄娟[1] 周仁超[1] 李泽恩[1] 徐诗如[1] 阮婷[1] 庞启华[1] 

机构地区：[1]华南师范大学生命科学学院,广州510631

出　　处：《生态学报》2015年第10期3204-3213,共10页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(31070003);广州市科技计划项目(2013J4100050)

摘　　要：以道地产地高良姜为材料,对该药用植物内生细菌种群组成、组织分布及其促生潜力进行了研究。采用组织块分离法,从高良姜根、根茎、茎和叶4个组织共计分离得到细菌136株,分属于16个16S r DNA基因型、12个细菌属、15个细菌种,其中,芽孢杆菌、甲基杆菌分别为其最、次优势种群。各种群分离比随组织不同而异;采用Hha I消化的末端限制性片段多态性(TRFLP)免培养方法,从高良姜4个组织共计检测到36个不同的末端限制性酶切片段(T-RFs)。种群对应分析表明其免培养内生细菌群体主要包括芽孢杆菌、甲基杆菌等高抗性细菌,海洋螺菌、红杆菌、交替假单胞菌等海洋细菌及热带根瘤菌、沼泽考克氏菌等热带相关细菌,说明高良姜内生细菌群体与其宿主植物生长环境密切相关。不同组织其总T-RFs数目、优势T-RFs及其对应细菌种群明显不同;在所分离的细菌菌株中,36.36%、51.52%、54.55%和27.27%的菌株分别显示了胞外几丁质酶、β-葡聚糖酶,生长素及其1-氨基环丙烷-1-羧酸(ACC)脱氨酶产生能力。其中,泛菌L-2、芽孢杆菌S-16所测4个指标均为阳性,热带根瘤菌菌株R-1和R-3显示了较高的ACC脱氨酶及生长素产生能力,这些菌株是促生菌剂的良好候选。Alpinia officinarum Hance, which is a species of the family Zingiberaceae, is a perennial medicinal plant that is mainly distributed in the tropical and subtropical regions of Southeast Asia. Although the endophytic communities of various crop plants and several medicinal plants have been investigated, no publications have reported research related to the endophytes of A. officinarum Hance. In the present study, a culture-dependent method combined with a terminal-restriction fragment length polymorphism （T-RFLP） technique-based culture-independent method were applied to assess the diversity of the culturable bacterial endophyte communities and total bacterial endophyte communities of A. officinarum Hance. Using the culture-dependent isolation method, 136 bacterial isolates were obtained from the internal tissues of root, rhizome, stem, and leaf of healthy, 4-year-old A. officinarum Hance plants collected from Longtang Town in Xuwen County, Guangdong Province, China. We selected the A. officinarum Hance plants growing in this area because the quality and pharmacological effects of these medicinal plants growing in this area are traditionally considered to be significantly higher than those of any other areas of China. Based on 16S rDNA sequence analysis, the bacterial isolates represented 16 distinct 16S rDNA gene types, 12 distinct bacterial genera, and 15 distinct bacterial species. The most dominant bacterial population was the genus Bacillus, followed by Methylobacterium. The culturable bacterial communities that were obtained from the different tissues of the medicinal plant were significantly different, and several bacterial populations displayed some level of tissue preference in the host plant. Using the T-RFLP-based culture-independent method, 36 distinct Terminal- Restriction Fragments （T-RFs） were detected from the amplified, HhaI mono-digestion-targeted 16S rDNA gene sequences from internal tissues of the root, rhizome, stem, and leaf of A. officinarum Hance plants collected from the same sites

关 键 词：道地高良姜 内生细菌 分离培养 T-RFLP 植物促生作用 

分 类 号：S567[农业科学—中草药栽培] S182[农业科学—作物学]