腭裂相关基因TTF-2转基因小鼠模型的制备过程  

Generation of cleft palate relevant gene TTF-2 transgenic mice

作　　者：黄磊[1] 庄庆[1] 石冰[2] 蒙田[2] 郑谦[2] 

机构地区：[1]广州市第一人民医院口腔科,广州510180 [2]口腔疾病国家重点实验室.四川大学华西口腔医学院唇腭裂外科,成都610000

出　　处：《广州医药》2015年第3期1-4,共4页Guangzhou Medical Journal

基　　金：国家自然科学基金(30171020)

摘　　要：目的建立表达TTF-2的转基因小鼠。方法利用显微注射技术把线性化表达载体p BROAD3-TTF-2注射到小鼠受精卵的雄原核中,建立TTF-2转基因小鼠。采用PCR、Southern印迹的方法对转基因小鼠进行鉴定。结果得到胎鼠68只和2只腭裂新生鼠,切鼠尾提取基因组DNA经PCR和Southern印迹方法检测发现13只转基因阳性胎鼠,包括2只腭裂新生鼠。腭裂小鼠出生后24小时死亡。结论获得了TTF-2转基因小鼠,表现型为腭裂,为腭裂发生机制的研究提供一种良好的动物模型。Objective To establish expressing TTF-2 transgenic mouse model. Methods The linear expression vector pBROAD3-TTF-2 containing TTF-2gene was microinjected into the male pronuclei of the fertilized mouse ovum the the model of TTF-2 transgenic mice was established.. The genotype of transgenic mice was identified by PCR and Southern blot. Results 68 embryos and 2 newborn mice with cleft palate were obtained for analysis. The genotype of the mice was determined by PCR and Southern blot using genomic DNA extracted from tail biopsies of the transgenic foetus. 13 positive TTF-2 transgenic mice was detected, which contained 2 mice with cleft palate. 2 transgenic mice with cleft palate died 24 hours after their birth. Conclusion The model of TTF-2 transgenic mice was established, which displayed a phenotype of cleft palate. It can be used as an animal model for the research of cleft palate.

关 键 词：转基因动物 显微注射 TTF-2 

分 类 号：R782.22[医药卫生—口腔医学] R-332[医药卫生—临床医学]