脂溶性HHRT与水溶性MMC、5-Fu对人Tenon's囊成纤维细胞抑制作用的研究  

Study on the inhibitory effects of Homoharringtonine,Mitomycin-C and 5-Fluorouracil and cultured Human Tenon's fibroblasts

作　　者：冷云霞[1] 张柳[1] 陈文俐[1] 黄雄飞[1] 杨为中[1] 

机构地区：[1]广州市第一人民医院,广州510180

出　　处：《广州医药》2015年第3期4-8,共5页Guangzhou Medical Journal

基　　金：广东省自然科学基金博士启动项目(S2013040012562);广州市科技局基金项目(11C33150706)

摘　　要：目的比较脂溶性高杉尖三酯碱(HHRT)与水溶新丝裂霉素C(MMC)和5-氟尿嘧啶(5-Fu)三种药物对人Tenon's囊成纤维细胞(HTFs)增殖率的抑制情况。方法采用冻存的人眼Tenon's囊成纤维细胞,常规复苏体外培养稳定后,接种于96孔板,分别加入不同浓度HHRT、MMC及5-Fu,继续培养;采用MTT法于加药后24h、48 h、72 h检测不同浓度HHRT、MMC及5-Fu对体外培养的人眼Tenon囊成纤维细胞增值率的抑制情况。结果一定浓度的HHRT、MMC及5-Fu,对HTFs的抑制作用呈现不同的剂量和时间依赖性,在药物浓度达到相应程度作用72 h后三者对HTFs的最大抑制率均可达70%以上,且组间对比差异有统计学意义。结论三种药物均对人眼Tenon's囊成纤维细胞增殖具有明显抑制作用,其最大抑制率均可达70%以上,三种药物对成纤维细胞抗增殖效应的时间浓度依赖性有所差别。Objective To investigate and compare the inhibitory effects of Mitomycin C （MMC）, 5-Fluorouraeil （5- Fu） and Homoharringtonine （HHRT） on cultured Human Tenon＇s fibroblasts （HTFs） proliferation. Methods Fibroblasts were recovered and cultured from cell bank. Fibroblasts were treated with different concentration of MMC, 5-Fu and HHRT for 48h and 72h respectively. The proliferation of fibroblasts was detected using MTT assay. Results Fibroblasts were cultured in MMC, 5-Fu or HHRT demonstrated a dose-dependent and time-dependent inhibition of fibroblasts proliferation. The maximum in- hibition rate was found when HTFs were treated with different concentration of MMC, 5-Fu or HHRT for 72h. And the three a- gents maximum inhibition rates were all more than 70%. The differences of proliferation in control and treated groups was statisti- cally significant （P 〈0.01 ） . Conclusion The three agents had inhibitory effect on HTFs. And the three agents maximum inhibition rates were all more than 70%. The inhibitory effects of MMC, 5-Fu and dexamethasone on fibroblast proliferationwas MMC 〉 HHRT 〉 5-Fu.

关 键 词：高三尖杉酯碱/HHRT 丝裂霉素C/MMC 5-氟尿嘧啶/5-Fu 人Tenon's囊成纤维细胞/HTFs 比色法/MTT 

分 类 号：R775[医药卫生—眼科]