木薯品种西选03快繁体系的建立（英文）  

Establishment of rapid propagation system for cassava variety Xixuan 03

作　　者：王燕[1] 杨美纯[1] 牛俊乐[1] 岑湘涛[1] 农艳丰[1] 

机构地区：[1]广西大学农学院,南宁530005

出　　处：《南方农业学报》2015年第4期539-543,共5页Journal of Southern Agriculture

基　　金：National Natural Science Foundation of China(31160300)

摘　　要：【目的】探讨木薯品种西选03快繁技术,为其种苗规模化生产提供技术支持。【方法】以水培幼茎腋芽为外植体,调查MS基本培养基中添加不同用量6-BA和PP333对外植体芽诱导、增殖培养和生根的影响。【结果】外植体采用0.1%KMNO4预处理10 min后经75%酒精20 s+0.1%Hg Cl2(加吐温-80)5 min的灭菌效果较好,污染率为14.0%,成活率达79.3%。采用MS+6-BA 0.05 mg/L培养基进行初代诱导效果较好,外植体培养15 d腋芽萌发率达90.0%,且萌发芽生长旺盛,茎秆粗壮。无菌腋芽茎段在MS+6-BA 0.1 mg/L培养基中继代增殖效果最佳,芽增殖倍数为4.6。MS+PP3330.3 mg/L培养基诱导生根效果较好,生根率100.0%,培养30 d平均根数为6.9,叶色墨绿,植株健壮。【结论】以幼茎腋芽为外植体的快繁体系可用于西选03种苗规模化生产。[Objective]The rapid propagation technique of cassava Xixuan 03 was studied in order to provide supports for its seedling production in a large scale. [Method]The hydroponic young axillary buds of cassava Xixuan 03 were used as explants for rapid propagation. The hormones （6-BA and PP333） with different concentrations were added in MS medium to investigate their effects on buds induction, multiplication and rooting induction. [ Result ]The results showed that, the treatment of pretreated with 0.1% KMNO4 for 10 min, followed by sterilized with 75% ethanol for 20 s and 0.1% HgCl2 solution with Tween-80 for 5 min, could get better sterilization effects on explants, its contamination rate was 14.0% and survival rate was 79.3%. As a result, the most suitable medium for buds induction of explants was MS＋ 6-BA 0.05 mg/L, the germination rate of axillary bud was 90.0% for inoculating 15 days later, and the sprouted buds grew strong. The optimum medium for multiplication culture of axillary bud was found as MS＋6-BA 0.1 mg/L with multiplication time of 4.6. MS medium added with PP333 0.3 mg/L was more effective for rooting culture with rooting rate of 100.0%, and the average roots was 6.9 after culturing for 30 days. The plantlets were strong with blackish green leaves. [Conclusion]The rapid propagation system by using axillary buds derived from young stem as explants, could be used for cassava Xixuan 03 seedlings production in a large scale.

关 键 词：木薯 西选03 快繁体系 规模化生产 

分 类 号：S533.035.3[农业科学—作物学]