乙型肝炎病毒X基因BCP双变异和CP聚集变异及其临床意义  

Analysis and clinical significance of the BCP double mutation and CP gathered variation in hepatitis B virus X gene

作　　者：高慧 何金花 崔美玲 刘志伟 梁紫甄 

机构地区：[1]广州市番禺区中心医院检验科,广东广州511400

出　　处：《海南医学》2015年第9期1304-1306,共3页Hainan Medical Journal

基　　金：广州市番禺区科技计划项目(编号:2012-Z-03-17)

摘　　要：目的探讨广州市番禺区133例乙型肝炎患者血清中HBV X基因区BCP双变异(A1762T/G1764A)和CP聚集变异情况及其临床意义。方法丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)采用速率法;ELISA和PCR法分别检测HBe Ag和HBV DNA拷贝数;并以PCR方法扩增HBV X基因,再进行测序,最后将结果分成四组(BCP双变异、CP聚集变异、BCP双变异合并CP聚集变异,无BCP双变异和CP聚集变异)进行综合比较分析。结果 (1)HBe Ag阳性在BCP双变异组中检出率最低(P<0.05);(2)四组HBV DNA拷贝数两两比较差异均无统计学意义(P>0.05);BCP双变异、CP聚集变异HBe Ag阳性组的HBV DNA拷贝数均高于HBe Ag阴性组(P<0.05);(3)四组ALT、AST异常例数检出率差异均无统计学意义(P>0.05)。结论乙型肝炎患者常规的相关指标检测对病情的评估尚不全面,在条件许可情况下,对HBV X基因区的BCP双变异(A1762T/G1764A)和CP聚集变异的常见突变位点进行检测,并结合其他临床资料进行综合分析可做出较正确的诊断。Objective To investigate the BCP double mutation （A1762T/G1764A） and CP gathered varia- tion of serum hepatitis B virus （HBV） X gene region and their clinical significance in 133 patients with hepatitis B from Panyu district of Guangzhou. Methods Alanine aminotransferase （ALT）, aspartate aminotransferase （AST） were tested by velocity method. ELISA and PCR were used to detect HBeAg and HBV DNA copy number respectively. The HBV X gene was amplified by PCR and then sequenced. In order to make a comprehensive comparative analysis, the results were divided into four groups： BCP double mutation, CP gathered variation, BCP double mutation merge CP gathered variation, and no mutation. Results （1） The positive rate of HBeAg in BCP double mutant group was the lowest （P〈0.05）. （2） Four groups of HBV DNA copy pairwise comparisons showed no statistically significant difference （P〉0.05）. The copy number of HBV DNA BCP double mutation and accumulation of CP mutation in HBeAg positive group were higher than that in HBeAg negative group （P〈0.05）. （3） The abnormal rates of ALT and AST from the four groups were not statistically different （P〉0.05）. Conclusion To assess the disease of patients with hepatitis B by routine testing of the related indicators is not comprehensive. With permission, combining the common mutations of BCP double mutation （A1762T/G1764A） and CP gathered variation of HBV X gene region with clinical information can help the doctors make more accurate diagnoses.

关 键 词：乙型肝炎病毒 测序 X基因 BCP双变异 CP聚集变异 

分 类 号：R512.62[医药卫生—内科学]