黑果枸杞内生真菌RER4次生代谢产物活性研究  

Antimicrobial activity of the secondary metabolites of endophytic fungi RER4 fromLycium ruthenicum

作　　者：杨秀芳[1] 王鹏飞[1] 马养民[1] 贾强强[1] 

机构地区：[1]陕西科技大学化学与化工学院教育部轻化工助剂化学与技术重点实验室,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2015年第3期125-129,共5页Journal of Shaanxi University of Science & Technology

基　　金：教育部高等学校博士学科点专项基金科研项目(20116125110001)

摘　　要：采用96孔板倍半稀释法对黑果枸杞内生真菌RER4次生代谢产物中分离纯化得到的11个化合物的抑菌活性进行研究,测定获得了化合物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌、枯草芽孢杆菌、尖孢镰孢菌、小麦赤霉病菌、苹果树腐烂病菌、番茄灰霉病菌的最小抑菌浓度(MIC).结果表明,黑果枸杞内生真菌RER4次生代谢产物中9个化合物对细菌和真菌有不同程度的抑制作用,其中尿黑酸内酯、2,5-二羟基苯乙酸甲酯对测试菌均表现出良好的抑制作用,pyranonigrins A和asperpyrones A对细菌生长有较好的抑制作用,5-hydroxymethylfuran-3-carboxylic acid对革兰氏阳性菌有较好的抑制作用,尿黑酸内酯、2,5-二羟基苯乙酸甲酯、pyranonigrins A对部分细菌的MIC达到7.8μg/mL.并对分离得到的化合物尿黑酸内酯、2,5-二羟基苯乙酸甲酯、pyranonigrins A、rubrofusarin B、asperpyrones A的抗氧化活性进行研究,采用DPPH法测定自由基清除率,结果表明尿黑酸内酯和2,5-二羟基苯乙酸甲酯具有良好的抗氧化性,抗氧化能力由强到弱顺序为尿黑酸内酯>2,5-二羟基苯乙酸甲酯>pyranonigrins A>asperpyrones A>rubrofusarin B.从测试结果分析了化合物与抗氧化活性的构效关系.The Minmum Inhibiting Concentration （MIC） of 11 compounds isolated from the Lycium ruthenicurn RER4 fungal secondary metabolites, were measured by microdilution method on 96-well plates against E. coli, P. aeruginosa ,S. aureus,B, subtilis ,F. oxysporium, G. saubinerii,C, rnandshurica, B. cirerea. The results showed that 9 compounds exhibited different inhibition to bacterial and fungal growth. 5-hydroxybenzofuran-2 （3H）-one and methyl 2-（2,5-dihydroxyphenyl）acetate shown better inhibition to both bacteria and fungi, pyranonigrins A and asperpyrones A exhibited better bacterial growth inhibition,5-hydroxy- methylfuran-3-carboxylic acid shown a special interesting to Gram-negative bacteria. 5- hydroxybenzofuran-2（3H）-one, methyl 2-（2, 5-dihydroxyphenyl and pyranonigrins A1 had notable inhibiting microorganism activity to bacteria with the MIC7.8μg/mL. The antioxidant effects of compounds 5-hydroxybenzofuran-2 （3H）-one, methyl 2-（2,5-dihydroxyphenyl） acetate, pyranonigrins A, rubrofusarin B, asperpyrones A were tested. The DPPH radicalscavenging capacity was measured against various concentrations with ascorbic acid as contrasts. The result of antioxidant activity test showed that the antioxidant activity of 5- hydroxybenzofuran-2（3H）-one and methyl 2-（2, 5-dihydroxyphenyl）acetate was equal to vitamin C for DPPH. Form strong to weak ordering 5-hydroxybenzofuran-2 （3H）-one〉methyl 2-（2, 5-dihydroxyphenyl）acetate）pyranonigrins A）asperpyrones A〉rubrofusarin B.

关 键 词：内生真菌 黑果枸杞 代谢产物 抑菌活性 抗氧化性 构效关系 

分 类 号：R932[医药卫生—生药学]