α-蒎烯对黄粉虫体内3种保护酶活性的影响  

Effects of α-pinene on Three Enzymes of Tenebrio molitor

作　　者：王慧[1,2] 马玲[1] 孟庆坤 崔荣林 

机构地区：[1]东北林业大学林学院林木遗传育种与生物技术教育部重点实验室,黑龙江哈尔滨150040 [2]黑龙江省七台河市林业局,黑龙江七台河154600

出　　处：《安徽农业科学》2015年第18期125-127,共3页Journal of Anhui Agricultural Sciences

基　　金：国家林业公益性行业科研专项(201204501)

摘　　要：[目的]探究植物挥发性化合物α-蒎烯的杀虫活性与作用机理。[方法]采用熏蒸法测定α-蒎烯对黄粉虫幼虫的急性毒性,并测定处理不同时间点其对黄粉虫体内3种酶活性的影响。[结果]α-蒎烯对黄粉虫幼虫12、24、36和48 h的致死中浓度(LC50)分别为101.434、67.518、57.810和44.120μg/L。用亚致死浓度(LC20)、LC50的α-蒎烯对黄粉虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)12、24、36、48 h的影响存在差异,但均表现出抑制功能。综合时间-剂量效应及酶受到抑制的程度来看,3种酶对α-蒎烯胁迫的敏感性从大到小依次为SOD、POD、CAT。[结论]α-蒎烯对黄粉虫有较高的生物活性,能有效干扰其体内酶系统,扰乱其正常生理代谢,表现出较高的毒杀效果。[ Objective ] The aim was to insecticidal activity and toxicity mechanism of the plant volatile organic compounds of α-pinene. [ Method] The acute toxicity of α-pinene against larvae of T. molitor was tested under the laboratory conditions with the method of fumigation, and its effects on the in vivo activities of the three kinds of enzymes at different time points. [ Result] The acute toxicity LC50 values of α-pi- nene against larvae of T. molitor in 12, 24, 36 and 48 hours post treatment were 101. 434, 67. 518, 57. 810 and 44. 120 μg/L, respectively. Though the LC20 and LC50 of α-pinene affected differently to superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） in T. moli- tor body in 12, 24, 36, 48 hours, all of them showed inhibition effect. Combining time-dose effect and the inhibition degree of the enzyme, it is concluded that the stress sensitive three biochemical parameters to α-pinene was SOD 〉 POD 〉 CAT. [ Conclusion] α-pinene had high bi- ological activity on T. molitor, which could effectively interference the enzymes, disrupt the normal physiological metabolism and show high toxicity effect.

关 键 词：Α-蒎烯 黄粉虫 作用机理 

分 类 号：S482.3[农业科学—农药学]