糖原合成酶激酶3β基因多态性与重性抑郁障碍的相关性研究  

Association study of glycogen synthase kinase-3β gene polymorphisms and major depressive disorder

作　　者：赵永萍[1,2] 仇玉莹[1,2] 刘莹[1,2] 秦琴[1,2] 张静[1,2] 贾琼[1,2] 程世翔[3] 张赛[3] 李洁[1,2] 

机构地区：[1]天津医科大学精神医学教研室,天津300070 [2]天津市安定医院 [3]武警部队脑创伤与神经疾病研究所,中国武警脑科医院

出　　处：《中华行为医学与脑科学杂志》2015年第4期315-318,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：天津市科技计划项目（09ZCZDSF04600）;天津市卫生行业重点攻关项目（13KG118）

摘　　要：目的 探讨糖原合成酶激酶3β（Glycogen synthase kinase-3β,GSK-3β）基因rs334558位点多态性与中国汉族人群重性抑郁障碍（major depressive disorder,MDD）之间的关系.方法 以250例MDD患者及300例健康对照作为研究对象,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术测定研究对象的GSK-3β基因rs334558位点的CC/CT＋TT基因型及等位基因频率分布.运用SPSS1Z0进行数据分析.结果 （1）病例组GSK-3β rs334558位点基因分布（CC/CT＋TT基因型频率分别为38.4％和61.6％,C、T等位基因频率分别为63.2％和36.8％）与对照组（CC/CT＋TT基因型频率分别为43.0％和57.0％,C、T等位基因频率分别为67.2％和32.8％）相比,均差异无统计学意义（x2=1.194,P=0.275;x2=1.895,P=0.169）;（2）在女性中,病例组GSK-3β rs334558位点基因分布（CC/CT＋TT基因型频率分别为37.9％和62.1％,C、T等位基因频率分别为63.5％和36.5％）与对照组（CC/CT＋ TT基因型频率分别为51.0％和49.0％,C、T等位基因频率分别为72.4％和27.6％）相比,均差异有统计学意义（x2=6.499,P=0.011;x2=6.858,P=0.009）,女性中携带T等位基因的人群患MDD的危险度是携带C等位基因的1.504倍（OR=1.504,95％ CI=1.107～2.043）;（3） GSK-3β基因rs334558位点CC/CT＋TT基因型、等位基因与女性MDD抑郁症状的严重程度的关系中均差异无统计学意义（x2=0.519,P=0.471;x2=0.199,P=0.656）.结论 GSK-3β基因rs334558位点多态性可能与中国女性人群MDD发病有关,且携带T等位基因可能增加女性患MDD的危险性,但该基因位点多态性对女性MDD的抑郁严重程度无影响.Objective To explore the association between glycogen synthase kinase-3β （GSK-3β） rs334558 gene polymorphism and major depressive disorder （MDD） in Han Chinese.Methods Genotypes and alleles frequency of GSK-3β rs334558 polymorphism were examined in 250 patients and 300 healthy controls by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.SPSS 17.0 statistical software was used for statistical analysis.Results （1） GSK-3β rs334558 locus of CC,CT＋TT genotypes and alleles frequency distribution between patient group （CC,CT＋TT genotypes：38.4%,61.6%.C,T alleles：63.2%,36.8%） and control group （CC,CT＋TT genotypes：43.0%,57.0%.C,T alleles：67.2%,32.8%） had no significant difference （x2 =1.194,P=0.275.x2 =1.895 P=0.169）;（2） There were significant differences of rs334558 genotypes and alleles between patient group （CC,CT＋TT genotypes：37.9%,62.1%.C,T alleles：63.5%,36.5%） and control group （CC,CT＋TT genotypes：51.0%,49.0%.C,T alleles：72.4%,27.6%） in female （x2=6.499,P =0.011.x2 =6.858,P=0.009）.T allele was a risk factor for female MDD compared with C allele （OR=1.504,95% CI =1.107 ～ 2.043）;（3） There was no association between rs334558 polymorphism and MDD severity in female （x2=0.519,P=0.471.x2=0.199,P=0.656）.Conclusion This study suggests that GSK-3β rs334558 polymorphism may be associated with the onset of MDD among female,and T allele may be a risk factor.But there is no association between polymorphism and severity of female MDD.

关 键 词：重性抑郁障碍 糖原合成酶激酶3Β 基因多态性 

分 类 号：R749.4[医药卫生—神经病学与精神病学]