半枝莲体外抗子宫内膜癌活性的研究  被引量:6

Study on the growth inhibitory effect of Scutellariae Barbatae on endometrial cancer

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作  者:满其倩 左琳[1] 蒋海强[1] 巩丽丽[1] 容蓉[1] 吕青涛[1] 

机构地区:[1]山东中医药大学药学院,济南250355

出  处:《现代妇产科进展》2015年第4期269-272,共4页Progress in Obstetrics and Gynecology

基  金:国家自然科学基金资助项目(No:81173614)

摘  要:目的:研究半枝莲不同极性部位体外抗子宫内膜癌细胞Ishikawa的活性作用。方法:采用MTT法检测半枝莲不同极性部位对子宫内膜癌细胞Ishikawa的增殖抑制作用;采用平板克隆形成实验检测活性最强部位对Ishikawa细胞克隆形成能力的影响;应用流式细胞仪检测活性最强部位对Ishikawa细胞周期分布的影响。结果:半枝莲不同极性部位对子宫内膜癌细胞Ishikawa均有不同程度的抑制作用,其中氯仿部位的抑制作用最强(IC50=146.494μg/ml)。随着供试药物浓度的升高,细胞克隆形成能力降低;半枝莲氯仿部位对Ishikawa细胞表现为S期、G2/M期阻滞作用。结论:半枝莲氯仿部位是半枝莲抗子宫内膜癌细胞Ishikawa的最主要有效部位。Objective:To investigate the growth inhibitory effect of the different polarity extracts of Scutellariae Barbatae on the human endometrial cancer cell line Ishikawa. Methods:Inhibition of cell proliferation was measured by MTT assay. Plate clone experiment was used to detect the clone forming ability of Ishikawa cells after treatment with the tested medicine. Flow cytometry was used to measure the cell cycle distributions of Ishikawa cells. Result:The differ-ent polarity extracts inhibited the proliferation of Ishikawa cells. The IC50 value of the most ac-tive extract was 146. 494μg/ml. Clone forming ability of cells decreased as medicine concentra-tion increased. The most active extract induced S-phase and G2/M-phase arrest. Conclusions:The chloroform extract of Scutellariae Barbatae had strongest inhibitory effect on Ishikawa cells.

关 键 词:半枝莲 ISHIKAWA细胞 MTT法 平板克隆形成实验 细胞周期 

分 类 号:R737.33[医药卫生—肿瘤]

 

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