EphA7在炎症状态下人牙髓组织中的表达和意义  

Expression and significance of EphA7 in inflamed human dental pulp

作　　者：赵俊杰[1] 兰卫东[1] 董迎春[1] 

机构地区：[1]南京市口腔医院,南京大学医学院附属口腔医院,江苏南京210008

出　　处：《牙体牙髓牙周病学杂志》2015年第4期197-200,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助课题(81100768);江苏省南京市医学科技发展专项资金资助课题(YKK11040;QRX11123)

摘　　要：目的:探讨Eph A7基因在牙髓的炎症反应和牙源性疼痛中的作用及意义。方法:收集健康牙髓、具有疼痛症状的牙髓炎牙髓和无疼痛症状的牙髓炎牙髓各18例,分别采用免疫组化染色和Western印迹法检测Eph A7蛋白在不同状态牙髓组织中的表达水平。结果:免疫组化染色显示:Eph A7在健康牙髓中仅阳性表达于血管内皮细胞和成牙本质细胞;而在炎症牙髓中,Eph A7的表达显著升高,除阳性表达于上述细胞外,同时还阳性表达于成纤维细胞、炎症细胞以及神经纤维组织中。Western印迹法检测结果显示:与健康牙髓相比,牙髓炎牙髓中Eph A7蛋白的表达水平显著增高(P<0.05),具有疼痛症状的牙髓中Eph A7蛋白的表达水平显著高于无疼痛症状的牙髓(P<0.05)。结论:Eph A7基因可能是反映牙髓组织炎症活动和疼痛状态的一个标记物。AIM：To investigate the role of EphA7 gene in pulp inflammation and odontogenic pain. METHODS：18 healthy controls,18 asymptomatic and 18 symptomatic irreversible pulpitis human dental pulps were included in the study.The protein expression of EphA7 was detected by immunohistochemistry and western blotting re-spectively.RESULTS：In healthy samples,immunohistochemical staining showed that positive EphA7 expression was only in vascular endothelial cells and odontoblasts.In the inflammatory pulps ,EphA7 expression increased significant-ly.In addition to vascular endothelial cells and odontoblasts,EphA7 positive staining was also detected in fibroblasts, inflammatory cells and nerve fiber tissues.Western blotting analysis showed that EphA7 expression increased signifi-cantly in pulpitis samples compared with healthy controls.In symptomatic irreversible pulpitis samples,EphA7 expres-sion was significantly higher than those in asymptomatic ones.CONCLUSION：EphA7 gene may be a marker reflec-ting inflammatory and pain state of human dental pulp.

关 键 词：EPHA7 牙髓 牙髓炎 牙源性疼痛 

分 类 号：R780.2[医药卫生—口腔医学]