α-磷酸三钙/胶原复合体与自体骨骨修复能力比较  

Alpha-tricalcium phosphate / atelocollagen sponge composite versus autogenous bone for bone repair efficacy

作　　者：李佩祺 吴补领[1] 高杰[1] 方一如 松本尚之 桥本典也 廖文[3,5] 

机构地区：[1]南方医科大学南方医院牙体牙髓病-国家临床重点专科 [2]南方医科大学口腔医学院,广东广州510515 [3]大阪齿科大学大学院齿科 [4]大阪齿科大学齿科 [5]四川大学口腔医学国家重点实验室,四川成都610041

出　　处：《牙体牙髓牙周病学杂志》2015年第4期223-228,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金项目(81371137)

摘　　要：目的:探讨α-磷酸三钙/胶原复合体修复大鼠头盖骨骨缺损能力。方法:选用8周龄SD大鼠28只,随机分为自体骨组(n=12)、α-磷酸三钙/胶原复合材料组(n=12)与空白组(n=4)。自体骨组和材料组于术后4、6、8周3个时间点各处死大鼠4只;空白组大鼠在8周全部处死。分别进行mirco-CT扫描分析,HE、TRAP和ALP染色,比较各组修复骨缺损效果。结果:8周时α-磷酸三钙/胶原复合物和自体骨的骨体积分数分别为(69.7±3.95)%和(42.475±4.38)%(P<0.05);随着时间延长,α-磷酸三钙/胶原复合材料逐渐被吸收,同时骨缺损亦逐渐修复;自体骨缺损部在6周后出现大量破骨细胞,大面积移植骨与新生骨被吸收,而α-磷酸三钙/胶原复合体修复骨缺损中较少发现破骨细胞。结论:α-磷酸三钙/胶原复合体具有较好骨缺损修复能力,且材料易于吸收。AIM：To study the effects ofα-tricalcium phosphate（α-TCP）/atelocollagen sponge composite in the repair of calvarial defects in rats.METHODS：Bone defects （diameter,8 mm;depth,1 .0 mm）were created in 28 male SD rats aged 8 weeks .The rats were divided into 3 groups.Inα-TCP group（n=12）the bone defects were filled with α-TCP /atelocollagen sponge composite,in positive control group （n=12）with autogenous bone,in blank control group （n=4）with nothing.The rats in α-TCP and positive control groups were euthanized at 4,6,and 8 weeks after surgery respectively（n=4）.The rats in blank control group （n=4）were euthanized 8 weeks after surger-y.The calvarial bone was scanned by micro-CT.HE TRAP and ALP staining were performed for the evaluation of new bone formation.RESULTS：Micro-CT images at 8 weeks after surgery revealed that,in α-TCP and positive control groups the bone volume was as （69.7 ±3.95）%and （42.475 ±4.38）%respectively（P〈0.05）.Histological anal-ysis revealed that in α-TCP group new bone contiguous with original bone was observed and the composite was ab-sorbed.In positive control group lot of osteoclasts and absorption of the transplanted autogenous bone were observed.In blank control group little new bone was observed at the boundary of the deffects.CONCLUSION：α-TCP /atelocolla-gen sponge composite is effective in the repair of bone defect.

关 键 词：临界性骨缺损 α-磷酸三钙/胶原复合体 自体骨 

分 类 号：R783.1[医药卫生—口腔医学]